Detailed information of TA system TA186218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 41993..42527
• Replicon: plasmid p5512.56-2-CTXM14
• Accession: NZ_CP067389
• Organism: Klebsiella grimontii strain 5512.56

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6P1V8P2
• Locus tag: JJJ11_RS00885
• Protein ID: WP_000143805.1
• Coordinates: 42237..42527 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7I6XZR7
• Locus tag: JJJ11_RS00880
• Protein ID: WP_000104338.1
• Coordinates: 41993..42247 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJJ11_RS00860 (JJJ11_00860)	37348..37797	-	450	WP_045911952.1	hypothetical protein	-
JJJ11_RS00865 (JJJ11_00865)	38047..39876	-	1830	WP_025760371.1	hypothetical protein	-
JJJ11_RS00870 (JJJ11_00870)	40506..41039	+	534	WP_224019189.1	hypothetical protein	-
JJJ11_RS00875 (JJJ11_00875)	41126..41836	+	711	WP_059514180.1	tyrosine-type recombinase/integrase	-
JJJ11_RS00880 (JJJ11_00880)	41993..42247	+	255	WP_000104338.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
JJJ11_RS00885 (JJJ11_00885)	42237..42527	+	291	WP_000143805.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JJJ11_RS00890 (JJJ11_00890)	42838..43437	-	600	WP_025760201.1	DUF2913 family protein	-
JJJ11_RS00895 (JJJ11_00895)	43457..44179	-	723	WP_025760202.1	NYN domain-containing protein	-
JJJ11_RS00900 (JJJ11_00900)	45282..46157	-	876	WP_094935765.1	incFII family plasmid replication initiator RepA	-
JJJ11_RS00910 (JJJ11_00910)	46460..46708	-	249	WP_058670686.1	replication regulatory protein RepA	-
JJJ11_RS00915 (JJJ11_00915)	46861..47415	-	555	WP_059509465.1	phospholipase D family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-14	-	1..92905	92905

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-47)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11463.41 Da
• Isoelectric Point: 10.2689

>T186218 WP_000143805.1 NZ_CP067389:42237-42527 [Klebsiella grimontii]
MTFNVEFDERAFKEWNKLEKTIREQFKKKLKKLQQNPYVESARLHGDLAGCFKIKLRASGFRLIYKVIDDEIVIWVVAVG
KREDEKAYETARKRLL

Nucleotide

• Length: 291 bp

>T186218 NZ_CP089272:3726351-3726737 [Escherichia coli O157:H7]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAAGCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9708.04 Da
• Isoelectric Point: 4.4247

>AT186218 WP_000104338.1 NZ_CP067389:41993-42247 [Klebsiella grimontii]
MSTIQIRVDDELKKDAYQAFEKLNLSPSDALRLFLRYVAENEKLPFAEVSVMVSEDDEDADILAVVRERLKNPARRIRVN
VDDL

Nucleotide

• Length: 255 bp

>AT186218 NZ_CP089272:3726727-3727056 [Escherichia coli O157:H7]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A6P1V8P2

Antitoxin

• AlphaFold DB: A0A7I6XZR7