Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5872..6397 Replicon plasmid p5512.56-2-CTXM14
Accession NZ_CP067389
Organism Klebsiella grimontii strain 5512.56

Toxin (Protein)


Gene name ccdB Uniprot ID A0A6S4YF27
Locus tag JJJ11_RS00700 Protein ID WP_025714517.1
Coordinates 5872..6177 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag JJJ11_RS00705 Protein ID WP_001568025.1
Coordinates 6179..6397 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJJ11_RS00670 (JJJ11_00670) 1559..2185 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
JJJ11_RS00675 (JJJ11_00675) 2182..2484 + 303 WP_071571079.1 hypothetical protein -
JJJ11_RS00680 (JJJ11_00680) 2948..3742 - 795 WP_004197635.1 site-specific integrase -
JJJ11_RS00685 (JJJ11_00685) 3940..4956 - 1017 WP_017899884.1 hypothetical protein -
JJJ11_RS00690 (JJJ11_00690) 4967..5281 - 315 WP_053389906.1 hypothetical protein -
JJJ11_RS00695 (JJJ11_00695) 5308..5703 - 396 WP_017899885.1 hypothetical protein -
JJJ11_RS00700 (JJJ11_00700) 5872..6177 - 306 WP_025714517.1 type II toxin-antitoxin system toxin CcdB Toxin
JJJ11_RS00705 (JJJ11_00705) 6179..6397 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JJJ11_RS00710 (JJJ11_00710) 6977..7546 + 570 WP_016162066.1 hypothetical protein -
JJJ11_RS00715 (JJJ11_00715) 7687..8715 + 1029 WP_001568023.1 Abi family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-14 - 1..92905 92905


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11562.25 Da        Isoelectric Point: 5.6906

>T186217 WP_025714517.1 NZ_CP067389:c6177-5872 [Klebsiella grimontii]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T186217 NZ_CP089272:3560402-3560689 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT186217 WP_001568025.1 NZ_CP067389:c6397-6179 [Klebsiella grimontii]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT186217 NZ_CP089272:3560211-3560402 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6S4YF27

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References