Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 56025..56761 Replicon plasmid pIncFIBK_IncFIIK
Accession NZ_CP067373
Organism Klebsiella pneumoniae strain BA30371

Toxin (Protein)


Gene name tacT Uniprot ID A0A8T5ZF70
Locus tag JI970_RS26000 Protein ID WP_004187044.1
Coordinates 56279..56761 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag JI970_RS25995 Protein ID WP_003026799.1
Coordinates 56025..56291 (+) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JI970_RS25980 (JI970_25970) 51497..52891 + 1395 WP_032439652.1 cytosine permease -
JI970_RS25985 (JI970_25975) 52903..54111 + 1209 WP_032439650.1 imidazolonepropionase -
JI970_RS25990 (JI970_25980) 54104..54913 + 810 WP_023329017.1 N-formylglutamate deformylase -
JI970_RS25995 (JI970_25985) 56025..56291 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
JI970_RS26000 (JI970_25990) 56279..56761 + 483 WP_004187044.1 GNAT family N-acetyltransferase Toxin
JI970_RS26005 (JI970_25995) 56968..58314 + 1347 WP_077254728.1 ISNCY family transposase -
JI970_RS26010 (JI970_26000) 58363..58761 + 399 WP_032422684.1 helix-turn-helix domain-containing protein -
JI970_RS26015 (JI970_26005) 58908..59018 - 111 Protein_58 IS3 family transposase -
JI970_RS26020 (JI970_26015) 59293..60229 + 937 Protein_59 ISNCY family transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / qnrB1 / tet(A) - 1..226169 226169


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(46-148)

Antitoxin

(6-82)


Sequences


Toxin        


Download         Length: 161 a.a.        Molecular weight: 17280.93 Da        Isoelectric Point: 8.7197

>T186216 WP_004187044.1 NZ_CP067373:56279-56761 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Download         Length: 483 bp

>T186216 NZ_CP089272:c3553450-3553295 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT186216 WP_003026799.1 NZ_CP067373:56025-56291 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT186216 NZ_CP089272:3553462-3553520 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References