Detailed information of TA system TA186215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 11118..11761
• Replicon: plasmid pIncFIBK_IncFIIK
• Accession: NZ_CP067373
• Organism: Klebsiella pneumoniae strain BA30371

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: JI970_RS25780
• Protein ID: WP_016236302.1
• Coordinates: 11345..11761 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: JI970_RS25775
• Protein ID: WP_001261282.1
• Coordinates: 11118..11348 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JI970_RS25750 (JI970_25740)	6835..7092	-	258	WP_023292103.1	hypothetical protein	-
JI970_RS25755	7734..8249	+	516	Protein_6	CSS-motif domain-containing protein	-
JI970_RS25760	8313..9149	+	837	WP_032439674.1	EAL domain-containing protein	-
JI970_RS25765 (JI970_25750)	9767..10198	-	432	WP_174805835.1	hypothetical protein	-
JI970_RS25770 (JI970_25755)	10739..11161	-	423	WP_046624301.1	hypothetical protein	-
JI970_RS25775 (JI970_25760)	11118..11348	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JI970_RS25780 (JI970_25765)	11345..11761	+	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
JI970_RS25785 (JI970_25770)	11835..13397	+	1563	WP_117259263.1	AAA family ATPase	-
JI970_RS25790 (JI970_25775)	13382..14404	+	1023	WP_032439672.1	helicase UvrD	-
JI970_RS25795 (JI970_25780)	14949..15857	+	909	WP_032439686.1	HNH endonuclease	-
JI970_RS25800 (JI970_25785)	16043..16393	-	351	WP_004187110.1	DUF305 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / qnrB1 / tet(A)	-	1..226169	226169

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T186215 WP_016236302.1 NZ_CP067373:11345-11761 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T186215 NZ_CP089272:c3334650-3334276 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT186215 WP_001261282.1 NZ_CP067373:11118-11348 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT186215 NZ_CP089272:c3335071-3334697 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3