Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5232780..5233405 Replicon chromosome
Accession NZ_CP067372
Organism Klebsiella pneumoniae strain BA30371

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag JI970_RS25360 Protein ID WP_019705794.1
Coordinates 5232780..5233163 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JI970_RS25365 Protein ID WP_004150355.1
Coordinates 5233163..5233405 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JI970_RS25345 (5230146) 5230146..5231048 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JI970_RS25350 (5231045) 5231045..5231680 + 636 WP_032430299.1 formate dehydrogenase cytochrome b556 subunit -
JI970_RS25355 (5231677) 5231677..5232606 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JI970_RS25360 (5232780) 5232780..5233163 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
JI970_RS25365 (5233163) 5233163..5233405 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
JI970_RS25370 (5233610) 5233610..5234527 + 918 WP_032430300.1 alpha/beta hydrolase -
JI970_RS25375 (5234542) 5234542..5235483 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JI970_RS25380 (5235528) 5235528..5235965 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
JI970_RS25385 (5235962) 5235962..5236822 - 861 WP_004146232.1 virulence factor BrkB family protein -
JI970_RS25390 (5236816) 5236816..5237415 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T186214 WP_019705794.1 NZ_CP067372:c5233163-5232780 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T186214 NZ_CP089272:3097454-3097561 [Escherichia coli O157:H7]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT186214 WP_004150355.1 NZ_CP067372:c5233405-5233163 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT186214 NZ_CP089272:c3097401-3097340 [Escherichia coli O157:H7]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References