Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4013135..4013754 Replicon chromosome
Accession NZ_CP067372
Organism Klebsiella pneumoniae strain BA30371

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag JI970_RS19550 Protein ID WP_002892050.1
Coordinates 4013536..4013754 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag JI970_RS19545 Protein ID WP_002892066.1
Coordinates 4013135..4013509 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JI970_RS19535 (4008288) 4008288..4009481 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JI970_RS19540 (4009504) 4009504..4012650 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
JI970_RS19545 (4013135) 4013135..4013509 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
JI970_RS19550 (4013536) 4013536..4013754 + 219 WP_002892050.1 HHA domain-containing protein Toxin
JI970_RS19555 (4013913) 4013913..4014479 + 567 WP_002892030.1 maltose O-acetyltransferase -
JI970_RS19560 (4014451) 4014451..4014591 - 141 WP_004147370.1 hypothetical protein -
JI970_RS19565 (4014612) 4014612..4015082 + 471 WP_002892026.1 YlaC family protein -
JI970_RS19570 (4015057) 4015057..4016508 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
JI970_RS19575 (4016609) 4016609..4017307 + 699 WP_002892021.1 GNAT family protein -
JI970_RS19580 (4017304) 4017304..4017444 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
JI970_RS19585 (4017444) 4017444..4017707 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T186209 WP_002892050.1 NZ_CP067372:4013536-4013754 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T186209 NZ_CP089272:2348191-2348294 [Escherichia coli O157:H7]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT186209 WP_002892066.1 NZ_CP067372:4013135-4013509 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT186209 NZ_CP089272:c2348332-2348061 [Escherichia coli O157:H7]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References