Detailed information of TA system TA186208

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2654413..2656001
• Replicon: chromosome
• Accession: NZ_CP067372
• Organism: Klebsiella pneumoniae strain BA30371

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A6T928
• Locus tag: JI970_RS13015
• Protein ID: WP_004176197.1
• Coordinates: 2654413..2655735 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JI970_RS13020
• Protein ID: WP_032417111.1
• Coordinates: 2655735..2656001 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JI970_RS13000 (2649469)	2649469..2651970	+	2502	WP_016831617.1	fimbria/pilus outer membrane usher protein	-
JI970_RS13005 (2651958)	2651958..2652962	+	1005	WP_004176196.1	fimbrial protein	-
JI970_RS13010 (2653104)	2653104..2654396	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
JI970_RS13015 (2654413)	2654413..2655735	-	1323	WP_004176197.1	type II toxin-antitoxin system HipA family toxin	Toxin
JI970_RS13020 (2655735)	2655735..2656001	-	267	WP_032417111.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JI970_RS13025 (2656266)	2656266..2656592	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
JI970_RS13030 (2656589)	2656589..2657089	-	501	WP_002904787.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
JI970_RS13035 (2657067)	2657067..2658446	-	1380	WP_117259192.1	nucleobase:cation symporter-2 family protein	-
JI970_RS13040 (2658792)	2658792..2659946	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
JI970_RS13045 (2659927)	2659927..2660850	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49111.41 Da
• Isoelectric Point: 7.5357

>T186208 WP_004176197.1 NZ_CP067372:c2655735-2654413 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T186208 NZ_CP089272:1085981-1086316 [Escherichia coli O157:H7]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9926.32 Da
• Isoelectric Point: 8.5912

>AT186208 WP_032417111.1 NZ_CP067372:c2656001-2655735 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETASAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT186208 NZ_CP089272:1085733-1085981 [Escherichia coli O157:H7]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A6T928