Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5251982..5252607 Replicon chromosome
Accession NZ_CP067370
Organism Klebsiella pneumoniae strain B9122

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JI969_RS25400 Protein ID WP_002882817.1
Coordinates 5251982..5252365 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JI969_RS25405 Protein ID WP_004150355.1
Coordinates 5252365..5252607 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JI969_RS25385 (5249348) 5249348..5250250 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JI969_RS25390 (5250247) 5250247..5250882 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JI969_RS25395 (5250879) 5250879..5251808 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JI969_RS25400 (5251982) 5251982..5252365 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JI969_RS25405 (5252365) 5252365..5252607 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
JI969_RS25410 (5252812) 5252812..5253729 + 918 WP_009484979.1 alpha/beta hydrolase -
JI969_RS25415 (5253743) 5253743..5254684 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JI969_RS25420 (5254729) 5254729..5255166 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
JI969_RS25425 (5255163) 5255163..5256023 - 861 WP_004146232.1 virulence factor BrkB family protein -
JI969_RS25430 (5256017) 5256017..5256616 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T186194 WP_002882817.1 NZ_CP067370:c5252365-5251982 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T186194 NZ_CP089270:c4615754-4615356 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT186194 WP_004150355.1 NZ_CP067370:c5252607-5252365 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT186194 NZ_CP089270:c4616050-4615757 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References