Detailed information of TA system TA186192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4767553..4768069
• Replicon: chromosome
• Accession: NZ_CP067370
• Organism: Klebsiella pneumoniae strain B9122

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R4YAY3
• Locus tag: JI969_RS23090
• Protein ID: WP_004178374.1
• Coordinates: 4767553..4767837 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A919M0P8
• Locus tag: JI969_RS23095
• Protein ID: WP_032434351.1
• Coordinates: 4767827..4768069 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JI969_RS23065 (4763036)	4763036..4763299	-	264	WP_014908078.1	PTS sugar transporter subunit IIB	-
JI969_RS23070 (4763429)	4763429..4763602	+	174	WP_032414379.1	hypothetical protein	-
JI969_RS23075 (4763605)	4763605..4764348	+	744	WP_002886905.1	MurR/RpiR family transcriptional regulator	-
JI969_RS23080 (4764705)	4764705..4766843	+	2139	WP_004186701.1	anaerobic ribonucleoside-triphosphate reductase	-
JI969_RS23085 (4767085)	4767085..4767549	+	465	WP_004222151.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
JI969_RS23090 (4767553)	4767553..4767837	-	285	WP_004178374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JI969_RS23095 (4767827)	4767827..4768069	-	243	WP_032434351.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
JI969_RS23100 (4768147)	4768147..4770057	-	1911	WP_110138999.1	PRD domain-containing protein	-
JI969_RS23105 (4770080)	4770080..4771234	-	1155	WP_002886900.1	lactonase family protein	-
JI969_RS23110 (4771300)	4771300..4772040	-	741	WP_002886899.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-68)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11155.97 Da
• Isoelectric Point: 10.3787

>T186192 WP_004178374.1 NZ_CP067370:c4767837-4767553 [Klebsiella pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T186192 NZ_CP089270:4299314-4299532 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9003.47 Da
• Isoelectric Point: 6.3340

>AT186192 WP_032434351.1 NZ_CP067370:c4768069-4767827 [Klebsiella pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGGKVSLDDL

Nucleotide

• Length: 243 bp

>AT186192 NZ_CP089270:4298914-4299288 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A6THG1