Detailed information of TA system TA186161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2761707..2762358
• Replicon: chromosome
• Accession: NZ_CP067365
• Organism: Lacticaseibacillus rhamnosus strain KF7

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K8Q4Y0
• Locus tag: JIO02_RS13065
• Protein ID: WP_005686631.1
• Coordinates: 2761707..2762090 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K8QH19
• Locus tag: JIO02_RS13070
• Protein ID: WP_005686632.1
• Coordinates: 2762110..2762358 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JIO02_RS13060	2760666..2761193	-	528	WP_005691221.1	QueT transporter family protein	-
JIO02_RS13065	2761707..2762090	-	384	WP_005686631.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JIO02_RS13070	2762110..2762358	-	249	WP_005686632.1	antitoxin	Antitoxin
JIO02_RS13075	2762470..2763609	-	1140	WP_005691215.1	alanine racemase	-
JIO02_RS13080	2763596..2763970	-	375	WP_005686634.1	holo-ACP synthase	-
JIO02_RS13085	2764139..2765647	-	1509	WP_005686635.1	DEAD/DEAH box helicase	-
JIO02_RS13090	2765921..2767309	-	1389	WP_193820308.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13757.03 Da
• Isoelectric Point: 10.9764

>T186161 WP_005686631.1 NZ_CP067365:c2762090-2761707 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T186161 NZ_CP089236:2032157-2032744 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGCGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAGTTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8936.13 Da
• Isoelectric Point: 4.4778

>AT186161 WP_005686632.1 NZ_CP067365:c2762358-2762110 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT186161 NZ_CP089236:2032811-2033665 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACACACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAACTGCTGCACGCGCG
CTATGTGGAGCAGCGCTTCGCGCCGCACGTGCACAGCAGCTTCGCCCTAGTCATCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGTGGCGGCGAGCACCTAGCCCCGCTGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGATGCCGGTTGGCTCTATCGCGGCTACTACCCGGAAATGGAACAGATCGACGGGATTCTCGACGAGCTGGAAAT
CCATCATGGCGGCGTGCCGCGTTTCGCCGACAGCGTGATCCACGACCAGCCCCTGGCACGCGCCTTCGTCCACTTGCACC
GCCTGCTGGCCGAGCATGCCGGCAGCCTGGAAACCCAGACCCGCTGGCGCGAGACGATGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCAGAAGCGCCGAAGGCCGGGCAGGAGCCGTTGGCGGTGGCCCGCGCCCGCGAACTGCTGGCGGCGCGGTT
GCTCGATCCGCCTTCTCTGGAGGAACTGGCGGCGGCGGTCAATCTGTCCCCGTTCCATTTTGCCCGGGTCTTCCGTCGCG
CCACCGGCATGCCGCCCCACGCCTGGCTCAGACAGCGCCGCCTGGAGCAGGCCAGGGCACTGCTCAAGAGCGGCCGGGCG
CCGCTGGAGGTGGCCCTGGAGCTGGGCTTCGCCGACCAGAGCCACCTCGGTCGCCAGTTCAAGCAGGCCTACGGGGTGGC
ACCGGGTGAATACCGCTCGGCCTGCGCGCGCTCGTCCTTGACGACATACGAATAA

Structures

Toxin

• AlphaFold DB: K8Q4Y0

Antitoxin

• AlphaFold DB: K8QH19