Detailed information of TA system TA186155

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 119404..120029
• Replicon: plasmid pTBH7P2
• Accession: NZ_CP067344
• Organism: Escherichia coli strain ECJXMCRH7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JJB06_RS24835
• Protein ID: WP_000911313.1
• Coordinates: 119404..119802 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: JJB06_RS24840
• Protein ID: WP_000450520.1
• Coordinates: 119802..120029 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB06_RS24800 (114738)	114738..116165	+	1428	WP_000146685.1	F-type conjugal transfer pilus assembly protein TraB	-
JJB06_RS24805 (116155)	116155..116745	+	591	WP_001376242.1	conjugal transfer pilus-stabilizing protein TraP	-
JJB06_RS24810 (116732)	116732..116929	+	198	WP_001324648.1	conjugal transfer protein TrbD	-
JJB06_RS24815 (116941)	116941..117192	+	252	WP_001038342.1	conjugal transfer protein TrbG	-
JJB06_RS24820 (117189)	117189..117704	+	516	WP_000809838.1	type IV conjugative transfer system lipoprotein TraV	-
JJB06_RS24825 (117839)	117839..118060	+	222	WP_001278689.1	conjugal transfer protein TraR	-
JJB06_RS24830 (118349)	118349..119395	+	1047	Protein_139	type IV secretion system DNA-binding domain-containing protein	-
JJB06_RS24835 (119404)	119404..119802	-	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
JJB06_RS24840 (119802)	119802..120029	-	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / floR / blaTEM-1A / mph(A) / dfrA12 / aadA2 / qacE / sitABCD	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..130221	130221

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T186155 WP_000911313.1 NZ_CP067344:c119802-119404 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T186155 NZ_CP089234:c5343325-5342942 [Klebsiella sp. KPN54798]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT186155 WP_000450520.1 NZ_CP067344:c120029-119802 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT186155 NZ_CP089234:c5343567-5343325 [Klebsiella sp. KPN54798]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: V0VCB2