Detailed information of TA system TA186151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 10094..10686
• Replicon: plasmid pTBH7P1
• Accession: NZ_CP067343
• Organism: Escherichia coli strain ECJXMCRH7

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1I9ZJC5
• Locus tag: JJB06_RS23860
• Protein ID: WP_000513532.1
• Coordinates: 10094..10342 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1I9ZJ49
• Locus tag: JJB06_RS23865
• Protein ID: WP_000681609.1
• Coordinates: 10339..10686 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB06_RS23825 (JJB06_23715)	5148..5771	-	624	WP_219360914.1	division plane positioning ATPase MipZ	-
JJB06_RS23830 (JJB06_23720)	6360..6689	+	330	WP_001542022.1	hypothetical protein	-
JJB06_RS23835 (JJB06_23725)	7090..7605	+	516	WP_001025393.1	J domain-containing protein	-
JJB06_RS23840 (JJB06_23730)	7709..8251	+	543	WP_001083904.1	hypothetical protein	-
JJB06_RS23845 (JJB06_23735)	8248..8502	+	255	WP_001230709.1	hypothetical protein	-
JJB06_RS23850 (JJB06_23740)	8499..8741	+	243	WP_000151456.1	hypothetical protein	-
JJB06_RS23855 (JJB06_23745)	9312..9605	+	294	WP_000673428.1	hypothetical protein	-
JJB06_RS23860 (JJB06_23750)	10094..10342	+	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	Toxin
JJB06_RS23865 (JJB06_23755)	10339..10686	+	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JJB06_RS25105	10774..11217	-	444	WP_023223351.1	hypothetical protein	-
JJB06_RS23875 (JJB06_23765)	11458..11757	+	300	WP_034169410.1	type II toxin-antitoxin system HicB family antitoxin	-
JJB06_RS23880 (JJB06_23770)	12017..12343	-	327	WP_000206697.1	hypothetical protein	-
JJB06_RS23885 (JJB06_23775)	12345..12668	-	324	WP_223666488.1	hypothetical protein	-
JJB06_RS25110	12645..12785	-	141	Protein_22	hypothetical protein	-
JJB06_RS23890 (JJB06_23780)	12805..13512	-	708	WP_000108794.1	hypothetical protein	-
JJB06_RS23895 (JJB06_23785)	13514..14266	-	753	WP_000797966.1	conjugal transfer protein TraL	-
JJB06_RS23900 (JJB06_23790)	14284..14685	-	402	WP_021575837.1	hypothetical protein	-
JJB06_RS23905 (JJB06_23795)	15053..15394	+	342	WP_000730722.1	plasmid mobilization relaxosome protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..61586	61586

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9378.03 Da
• Isoelectric Point: 10.4800

>T186151 WP_000513532.1 NZ_CP067343:10094-10342 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T186151 NZ_CP089234:2030690-2030792 [Klebsiella sp. KPN54798]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13206.97 Da
• Isoelectric Point: 4.8313

>AT186151 WP_000681609.1 NZ_CP067343:10339-10686 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Nucleotide

• Length: 348 bp

>AT186151 NZ_CP089234:c2030799-2030654 [Klebsiella sp. KPN54798]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: A0A1I9ZJC5

Antitoxin

• AlphaFold DB: A0A1I9ZJ49