Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3531082..3531919 Replicon chromosome
Accession NZ_CP067342
Organism Escherichia coli strain ECJXMCRH7

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag JJB06_RS17340 Protein ID WP_000227786.1
Coordinates 3531377..3531919 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag JJB06_RS17335 Protein ID WP_001297137.1
Coordinates 3531082..3531393 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB06_RS17310 (3526102) 3526102..3527049 + 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -
JJB06_RS17315 (3527071) 3527071..3529062 + 1992 WP_113403942.1 cytochrome o ubiquinol oxidase subunit I -
JJB06_RS17320 (3529052) 3529052..3529666 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
JJB06_RS17325 (3529666) 3529666..3529995 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
JJB06_RS17330 (3530007) 3530007..3530897 + 891 WP_000971336.1 heme o synthase -
JJB06_RS17335 (3531082) 3531082..3531393 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
JJB06_RS17340 (3531377) 3531377..3531919 + 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
JJB06_RS17345 (3531975) 3531975..3532910 - 936 WP_001297127.1 tetratricopeptide repeat protein -
JJB06_RS17350 (3533327) 3533327..3534691 + 1365 WP_001000978.1 MFS transporter -
JJB06_RS17355 (3534819) 3534819..3535310 - 492 WP_001138904.1 nucleotide binding protein YajQ -
JJB06_RS17360 (3535478) 3535478..3536389 + 912 WP_000705853.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T186145 WP_000227786.1 NZ_CP067342:3531377-3531919 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T186145 NZ_CP089227:5295607-5296014 [Nocardia asteroides]
GTGGCGAGTGACGAGTACGTACTCGACGGGTCGGCCGCCTCGACAGCGCTGCTGCGCAAGGACGCCGTCGGCATCGCCGT
CGGGCGGTTGATCGAGACCAGCACCTGCCACGCGCCGCATCTCATCGATGCGGAGGTCGGCAATGTGCTGCGTCGCCATG
AGCGCAAGGGCCTGGTCGACCCCGAGACCGCGACGATCGGCCTGCGGATGCTGGCGACGATGGTCGATCGGCGATATGCC
CACCAGGGCTGGCTCAGCGAGCAGGCGTGGCTGCTCCGCCACACGATCACGTTCTACGACGGCCTTTATGCCGCGCTCGC
GGCGCGACTCGATGTGCCGCTGCTCACCAGCGACGCGCGACTGAGTAAGGCACCGGGATTGCCGTGCCGGGTCGAAGTGA
TCGACTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT186145 WP_001297137.1 NZ_CP067342:3531082-3531393 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT186145 NZ_CP089227:5295378-5295617 [Nocardia asteroides]
ATGGCTACCATCCAGATCCGGGACATCCCCGACGAGGACGCCGAGGTGTTCCGCCGACGCGCCGAAGCCGAGGGCATGTC
GTTGCAGGCGTACATGCGACGGGAGTTGATCGCCGCCGCTCGTCGACGGTCGAAGACAGAGGCGTTGGCGGCTGTTCGCG
AAGCACTGGAACGTGATTCGAGCGCAGGCGCCGACCGCGAGTCGATCCTGAGCGCGTTGGACGACGCCCGTGGCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References