Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3496990..3497608 Replicon chromosome
Accession NZ_CP067342
Organism Escherichia coli strain ECJXMCRH7

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JJB06_RS17170 Protein ID WP_001291435.1
Coordinates 3497390..3497608 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JJB06_RS17165 Protein ID WP_000344800.1
Coordinates 3496990..3497364 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB06_RS17155 (3492079) 3492079..3493272 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JJB06_RS17160 (3493295) 3493295..3496444 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JJB06_RS17165 (3496990) 3496990..3497364 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JJB06_RS17170 (3497390) 3497390..3497608 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JJB06_RS17175 (3497780) 3497780..3498331 + 552 WP_000102564.1 maltose O-acetyltransferase -
JJB06_RS17180 (3498447) 3498447..3498917 + 471 WP_000136192.1 YlaC family protein -
JJB06_RS17185 (3499081) 3499081..3500631 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JJB06_RS17190 (3500673) 3500673..3501026 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JJB06_RS17200 (3501405) 3501405..3501716 + 312 WP_000409911.1 MGMT family protein -
JJB06_RS17205 (3501747) 3501747..3502319 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T186144 WP_001291435.1 NZ_CP067342:3497390-3497608 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T186144 NZ_CP089227:c5109791-5109510 [Nocardia asteroides]
GTGATCAGGTCATTCGGAGACCGGGACACTGAGCGTTTGTGGCATCGCGAGCATGTGAAGACGTTCGATTCCCGGATCCT
CCGGGTGGCGTTGCGCAAGCTCGCGATTTTGGACGCTGCGGTGTCGCTGGGCGAGCTTCGTGTTCCGCCGGGCAACCGGC
TGGAGGCGTTGAAGGGGAATCGCAAAGGGCAATACAGCCTTCGGATCAACGAGCAGTGGAGAATCTGTTTCGTCTGGACC
GATGCGGGACCCGAACAGGTGGCGATCGTGGACTACCACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT186144 WP_000344800.1 NZ_CP067342:3496990-3497364 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT186144 NZ_CP089227:c5109496-5109203 [Nocardia asteroides]
ATGGGCAAACTGGCTCCGGTGCATCCCGGTGAGGTGCTGAAGGAAGACTTCATCGAGCCGCTCGGCATCACTCAGCATCG
AGTCGCGGTGGCGATCGGTGTGCCCCCGCGCAGGATCAACGAGATCGTGCACGGCAAGCGGCGCATCACCGCGGACACGG
CCCTGCGCCTCGGGCGCTACTTCGGCACCAGCGACATCTTCTGGCTGAACCTCCAGAATCGCTACGACCTCGAGATCGAG
CGCGACGCGATCGGTGCCGCCTTGGCAGCCATTCAGCCGTTGCGCGTGGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References