Detailed information of TA system TA186143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2535984..2536622
• Replicon: chromosome
• Accession: NZ_CP067342
• Organism: Escherichia coli strain ECJXMCRH7

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: JJB06_RS12470
• Protein ID: WP_000813794.1
• Coordinates: 2536446..2536622 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JJB06_RS12465
• Protein ID: WP_001270286.1
• Coordinates: 2535984..2536400 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB06_RS12445 (2531136)	2531136..2532077	-	942	WP_001251313.1	ABC transporter permease	-
JJB06_RS12450 (2532078)	2532078..2533091	-	1014	WP_000220415.1	ABC transporter ATP-binding protein	-
JJB06_RS12455 (2533109)	2533109..2534254	-	1146	WP_113403949.1	ABC transporter substrate-binding protein	-
JJB06_RS12460 (2534499)	2534499..2535905	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
JJB06_RS12465 (2535984)	2535984..2536400	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JJB06_RS12470 (2536446)	2536446..2536622	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JJB06_RS12475 (2536844)	2536844..2537074	+	231	WP_000494244.1	YncJ family protein	-
JJB06_RS12480 (2537166)	2537166..2539127	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JJB06_RS12485 (2539200)	2539200..2539736	-	537	WP_219360859.1	DNA-binding transcriptional regulator SutR	-
JJB06_RS12490 (2539828)	2539828..2541003	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	sodB / espR1 / espR1	2293842..2618278	324436

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T186143 WP_000813794.1 NZ_CP067342:c2536622-2536446 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T186143 NZ_CP089225:3505924-3506295 [Nocardia gipuzkoensis]
CTGATGGAGTACCTGACGCTACCCGAGTTGCTGAATCTCGCGGAGCGGTTGGGCACTCCGACGGTGCGCGACTACGGCTT
GCTGGACTCCGCGCTGGCGCGCCCGCGGTCCAGCGTGTTCGGACAGGATGCCTACCCCGATGTATGGCAGAAGGCTGCCG
CGCTGATGGAGTCGATCGCCCGCAACCACGGCTTGGTCGACGGCAACAAGCGACTGGCGTGGTATGCCAGCTGGGTTTTC
CTGCAGATGAACGGTCATCGGCTGGACCCGCGCTTCGACGTCGACGACGCTGAGCAGTTCGTGCTCGATGTATGTCAGGG
TGTGTTGGACGTACCGGCAATCGCAGAGCATTTGGCGAAGTTCGAGAGCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT186143 WP_001270286.1 NZ_CP067342:c2536400-2535984 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT186143 NZ_CP089225:3505730-3505927 [Nocardia gipuzkoensis]
ATGGCCGGCCTGAACATTCGATTTTCCGACCAGGAACTGGGCGCCTTGCGCGAACGTGCGGAGGCTGAGGGCCGCAGTAT
GCAGCAGTTCGTCCACGATGCTGTGATCAAGGCGATCAACGAGCATTCCAGACTGTTCAACGAGGCAGCCGATCATGTCC
TGCGCGTCAGTGAGGAGCTGAACCGGAGGCTGGCCTGA

Structures

Toxin

• PDB: 6HPB