Detailed information of TA system TA186142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2451168..2452756
• Replicon: chromosome
• Accession: NZ_CP067342
• Organism: Escherichia coli strain ECJXMCRH7

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: JJB06_RS12100
• Protein ID: WP_113404043.1
• Coordinates: 2451434..2452756 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JJB06_RS12095
• Protein ID: WP_001301023.1
• Coordinates: 2451168..2451434 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB06_RS12095 (2451168)	2451168..2451434	+	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JJB06_RS12100 (2451434)	2451434..2452756	+	1323	WP_113404043.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
JJB06_RS12105 (2453053)	2453053..2453257	+	205	Protein_2377	fimbrial protein	-
JJB06_RS12110 (2453618)	2453618..2454328	+	711	WP_001195166.1	fimbria/pilus periplasmic chaperone	-
JJB06_RS12115 (2454370)	2454370..2457020	+	2651	Protein_2379	fimbrial biogenesis outer membrane usher protein	-
JJB06_RS12120 (2457034)	2457034..2457564	+	531	WP_000876763.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	sodB / espR1 / espR1	2293842..2618278	324436

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49317.90 Da
• Isoelectric Point: 8.2482

>T186142 WP_113404043.1 NZ_CP067342:2451434-2452756 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMVLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T186142 NZ_CP089225:3164155-3164412 [Nocardia gipuzkoensis]
ATGAAGGCCGTCTTCTCCGAAGCCGCGTGGGAAGAGTATTCGGCGTGGCTACAGCGCGACCGGCTCGTGCTGAAGAAGGT
CAATGTCCTGATCGAGGCAATCCTGGCCGATCCCTTCGAAGGCATCGGTAAGCCTGAGCCTTTGAAGCATGACTTGGCGG
GGTACTGGTCACGCCGGATCACGCAGGAGCATCGCATCGTCTACCGAATCGTCGACGACGCGGTTTGGATCGCCAAGGTA
GGAAAGCACTACGACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT186142 WP_001301023.1 NZ_CP067342:2451168-2451434 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

>AT186142 NZ_CP089225:3163886-3164158 [Nocardia gipuzkoensis]
TTGTACTACTGGCTGGGAGGAATGATGGCTTCGGCGATTACCGCGAGTGAAGCGCGCAGAAACTTGTTCCCCCTGATCAG
GATGGTCAATGAGGATCACGACACGATCGAGATCGTGTCGAAAGGGGGGAACGCCGTGCTGATGTCCAAGGAAGATTACG
ACTCGCTGATGACGACCGTGGAACTGTTGAGCGGTCGCGACGGGGCCCGCCTACGCCGGTCGATCCAGGAACTCGAGGGT
GGTGGCGGCGTCGAACGCGACCTCATCGAATGA