Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2393719..2394245 Replicon chromosome
Accession NZ_CP067342
Organism Escherichia coli strain ECJXMCRH7

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag JJB06_RS11745 Protein ID WP_000323025.1
Coordinates 2393958..2394245 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag JJB06_RS11740 Protein ID WP_000534858.1
Coordinates 2393719..2393958 (+) Length 80 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB06_RS11720 (2389559) 2389559..2390928 + 1370 WP_168728556.1 IS3-like element IS150 family transposase -
JJB06_RS11725 (2391125) 2391125..2392147 + 1023 Protein_2299 ISNCY family transposase -
JJB06_RS24975 (2392268) 2392268..2392417 + 150 WP_011443592.1 protein YdfW -
JJB06_RS11730 (2392854) 2392854..2393186 - 333 WP_001301033.1 FlxA-like family protein -
JJB06_RS11735 (2393389) 2393389..2393694 - 306 WP_071598992.1 protein YdfV -
JJB06_RS11740 (2393719) 2393719..2393958 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
JJB06_RS11745 (2393958) 2393958..2394245 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
JJB06_RS11750 (2394317) 2394317..2394472 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
JJB06_RS11755 (2394689) 2394689..2394940 + 252 WP_000980994.1 protein Rem -
JJB06_RS11760 (2395166) 2395166..2395327 + 162 Protein_2307 antitermination protein -
JJB06_RS11765 (2395605) 2395605..2395694 - 90 WP_120795389.1 hypothetical protein -
JJB06_RS11770 (2395749) 2395749..2395961 - 213 WP_000087756.1 cold shock-like protein CspF -
JJB06_RS11775 (2396262) 2396262..2396477 + 216 WP_000066484.1 cold shock-like protein CspB -
JJB06_RS11780 (2396841) 2396841..2397011 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
JJB06_RS11785 (2397231) 2397231..2397446 + 216 WP_000839590.1 phage lysis protein EssD -
JJB06_RS11790 (2397451) 2397451..2397762 + 312 WP_000189916.1 YdfR family protein -
JJB06_RS11795 (2397759) 2397759..2398292 + 534 WP_001092971.1 lysozyme -
JJB06_RS11800 (2398289) 2398289..2398786 + 498 WP_001071769.1 DUF2514 domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - sodB / espR1 / espR1 2293842..2618278 324436


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T186141 WP_000323025.1 NZ_CP067342:2393958-2394245 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T186141 NZ_CP089225:3007552-3007968 [Nocardia gipuzkoensis]
GTGACCGATTCCGAACAGATCGTTCTGGATGCCAGCGCGATGGTCGACATCCTTGTGCGCAATGACCGCACGGCCGCTGT
GATTTCGAGGATCACGACGACAGTCATGCATGTCCCGGCGCACTTCGACGCCGAAGTCCTGTCCGCACTCGGGCGACTGA
ACCGAGCGGGCGACTTGACCGATGGCGACGTCGAGACCGCACTGAGCCGCCTCGCCCGCGCGCCACTGACCCGGCATCCG
CTTCTGAACCTGACCAAAGGCGCTTGGGCTCGTCGTGCTGCGATCAGGCTGACCGATGCGCTCTATGTCGAACTCGCCGA
ACAACTGGACATGCCGCTGATCACCACGGACGGACGCCTCGCTCGAGCATCCTCGCGTGCCGAGGGAATCCGCGCTGCGG
CGGAACTCCGCGAATAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT186141 WP_000534858.1 NZ_CP067342:2393719-2393958 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT186141 NZ_CP089225:3007337-3007555 [Nocardia gipuzkoensis]
ATGACTCGGCTCAACGTTTACGTCCCCGATGAGCTGGCTGAGCGCGCCCGTGAGCGGGGGCTCAACGTGTCTGCGCTGAC
CCAGGCCGCGATTCTCGCGGCACTCGAAAAGAGCGCGACGTCGGCTTGGCTGGCTGAGTTCGACGACGACCGCCCCGAGG
TCTCGCACGAGGCCGTTCTTGCCGCCATCGATGCGGCGCGGGATGAGTTCGGCCGGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9


Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References