Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1095007..1095590 Replicon chromosome
Accession NZ_CP067342
Organism Escherichia coli strain ECJXMCRH7

Toxin (Protein)


Gene name mazF Uniprot ID A0A1V3W215
Locus tag JJB06_RS05305 Protein ID WP_000254741.1
Coordinates 1095255..1095590 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag JJB06_RS05300 Protein ID WP_000581937.1
Coordinates 1095007..1095255 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB06_RS05290 (1091346) 1091346..1092647 + 1302 WP_000046810.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
JJB06_RS05295 (1092695) 1092695..1094929 + 2235 WP_000226815.1 GTP pyrophosphokinase -
JJB06_RS05300 (1095007) 1095007..1095255 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
JJB06_RS05305 (1095255) 1095255..1095590 + 336 WP_000254741.1 endoribonuclease MazF Toxin
JJB06_RS05310 (1095661) 1095661..1096452 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
JJB06_RS05315 (1096680) 1096680..1098317 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
JJB06_RS05320 (1098405) 1098405..1099703 + 1299 WP_000036723.1 phosphopyruvate hydratase -
JJB06_RS05325 (1099763) 1099763..1100227 - 465 Protein_1044 YgcG family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1092695..1102670 9975


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12114.04 Da        Isoelectric Point: 8.2618

>T186131 WP_000254741.1 NZ_CP067342:1095255-1095590 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGSTKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T186131 NZ_CP089224:c3271942-3271544 [Mycobacterium ostraviense]
GTGAACGACTACGTCATTGATGCGTCGGCGCTAGTGCTGGCCTTGGCCGGCAAGACGAAAGCCGCCGACAGACTTCGGGC
CCGCCTGCCGGGCATGCGGCGACACGCGCCACAGCTGATCGACGCCGAAGTGGGCAACGTGCTGCGTCGCCATGAGCTTG
CGGGTCTCATCAGCACTAGGGAGGCTGAGACTGCGGCGCGTGTCGCCAGCTTGCTCATTGACGACCGATATCCGCACGTC
GGCGTACTTGCTCGACGCGCATGGACCCTGCGTCACACCATGACTTTCTACGACGCGCTCTACGTGGCACTCGCCAGCTA
CCTCGACATCCCACTACTCACCGGCGATGCGCGTCTTAGCCGCGCGCCGGGACTGATCTGCCAGACCGAACTGGTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT186131 WP_000581937.1 NZ_CP067342:1095007-1095255 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT186131 NZ_CP089224:c3272175-3271939 [Mycobacterium ostraviense]
ATGGCCACAATACAGATCCGCGAGATACCCGACGAAGCCTACGAGGTCATCCGCAAGCGCGCCCGTGCTGCGGGGCGCTC
CATCCAGTCCTACATGCGAGATTGGGTGATCCAGTTCGCGAGCCGTCCGACCACCGACGAAGCGCTGGCAGCCATGGAAG
CCGCGCGAGAAGCCAGCGAGACACCGGGGGCGACCAGGGAGTCCATCCTCACCGACTTGGCTGCGGACCGGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1V3W215

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References