Detailed information of TA system TA186129

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 851788..852623
• Replicon: chromosome
• Accession: NZ_CP067342
• Organism: Escherichia coli strain ECJXMCRH7

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: JJB06_RS04105
• Protein ID: WP_057109034.1
• Coordinates: 851788..852165 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: JJB06_RS04110
• Protein ID: WP_001285610.1
• Coordinates: 852255..852623 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB06_RS04075 (847793)	847793..848289	+	497	Protein_802	type VI secretion system contractile sheath small subunit	-
JJB06_RS04080 (848823)	848823..849116	+	294	WP_001110459.1	DUF1778 domain-containing protein	-
JJB06_RS04085 (849113)	849113..849601	+	489	WP_000622309.1	GNAT family N-acetyltransferase	-
JJB06_RS04090 (850442)	850442..851284	-	843	WP_039004748.1	DUF4942 domain-containing protein	-
JJB06_RS04095 (851369)	851369..851566	-	198	WP_023563519.1	DUF957 domain-containing protein	-
JJB06_RS04100 (851642)	851642..851791	-	150	Protein_807	DUF5983 family protein	-
JJB06_RS04105 (851788)	851788..852165	-	378	WP_057109034.1	TA system toxin CbtA family protein	Toxin
JJB06_RS04110 (852255)	852255..852623	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JJB06_RS04115 (852697)	852697..852918	-	222	Protein_810	DUF987 domain-containing protein	-
JJB06_RS04120 (853005)	853005..853481	-	477	WP_057109035.1	RadC family protein	-
JJB06_RS04125 (853497)	853497..853976	-	480	WP_057109036.1	antirestriction protein	-
JJB06_RS04130 (854242)	854242..855060	-	819	WP_001175153.1	DUF932 domain-containing protein	-
JJB06_RS04135 (855150)	855150..855383	-	234	WP_001278287.1	DUF905 family protein	-
JJB06_RS04140 (855389)	855389..856066	-	678	WP_001097368.1	hypothetical protein	-
JJB06_RS04145 (856185)	856185..857069	-	885	WP_000010392.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13934.86 Da
• Isoelectric Point: 6.8517

>T186129 WP_057109034.1 NZ_CP067342:c852165-851788 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLALNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T186129 NZ_CP089224:1978691-1979125 [Mycobacterium ostraviense]
ATGAAAATCGTCGACGCCAATGTCCTGCTGTATGCGGTTAATACCGCCAGCGAGCACCACAGCCCGTCCCTGCGCTGGCT
CGACGGCGCGCTATCCGGTGGCGACAGCATCGGATTCGCGTGGGTCCCCATGTTGGCGTTCGTGCGGCTAGCGACCAGGC
AGGGGTTGTTCCCCCGACCGCTGTCTCCGGAGGACGCGATCGGTCAGGTTGCCAATTGGCTGACCGCACCGAGCGCGGCA
CTAGTGAATCCGACCGCCCGACACGCCGACATCCTGGCGAGGATGCTGACACACGTGGGAACTGGCGCCAACCTCGTCAA
CGATGCTCACCTGGCCGCGCTCGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGCGATTTCGGGCGATTCGCCG
GCGTGCGCTGGGATCACCCGTCGGCGCTGCTGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT186129 WP_001285610.1 NZ_CP067342:c852623-852255 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT186129 NZ_CP089224:1978336-1978590 [Mycobacterium ostraviense]
ATGCGGACGACGGTGACGTTGGATGACGACGTCGAGCAGTTGGTGCGGCGCCGGATGGCCGAGCGGCAGGTGTCCTTCAA
GAAAGCGCTCAACGACGCGATTCGAGACGGCGCCTCCGGCCGGCCCGCGCCGCGCCGGTTCAGCACGCGCACGGCAGATT
TGGGTGTGCCCGCGGTCAATCTCGACCGGGCCTTGCAGCTTGCGGCCGAGATCGAGGACGAGGAACTCGTGCGTCGTCAG
CGACGTCGGTCATGA