Detailed information of TA system TA186128

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 848823..849601
• Replicon: chromosome
• Accession: NZ_CP067342
• Organism: Escherichia coli strain ECJXMCRH7

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: JJB06_RS04085
• Protein ID: WP_000622309.1
• Coordinates: 849113..849601 (+)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A7H9KCB5
• Locus tag: JJB06_RS04080
• Protein ID: WP_001110459.1
• Coordinates: 848823..849116 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB06_RS04055 (844587)	844587..845570	-	984	WP_162753665.1	KpsF/GutQ family sugar-phosphate isomerase	-
JJB06_RS04060 (846071)	846071..846331	-	261	WP_001325874.1	hypothetical protein	-
JJB06_RS04065 (846395)	846395..846730	-	336	WP_000789899.1	hypothetical protein	-
JJB06_RS04070 (846727)	846727..847200	-	474	WP_000143249.1	hypothetical protein	-
JJB06_RS04075 (847793)	847793..848289	+	497	Protein_802	type VI secretion system contractile sheath small subunit	-
JJB06_RS04080 (848823)	848823..849116	+	294	WP_001110459.1	DUF1778 domain-containing protein	Antitoxin
JJB06_RS04085 (849113)	849113..849601	+	489	WP_000622309.1	GNAT family N-acetyltransferase	Toxin
JJB06_RS04090 (850442)	850442..851284	-	843	WP_039004748.1	DUF4942 domain-containing protein	-
JJB06_RS04095 (851369)	851369..851566	-	198	WP_023563519.1	DUF957 domain-containing protein	-
JJB06_RS04100 (851642)	851642..851791	-	150	Protein_807	DUF5983 family protein	-
JJB06_RS04105 (851788)	851788..852165	-	378	WP_057109034.1	TA system toxin CbtA family protein	-
JJB06_RS04110 (852255)	852255..852623	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	-
JJB06_RS04115 (852697)	852697..852918	-	222	Protein_810	DUF987 domain-containing protein	-
JJB06_RS04120 (853005)	853005..853481	-	477	WP_057109035.1	RadC family protein	-
JJB06_RS04125 (853497)	853497..853976	-	480	WP_057109036.1	antirestriction protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (83-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17491.33 Da
• Isoelectric Point: 7.7535

>T186128 WP_000622309.1 NZ_CP067342:849113-849601 [Escherichia coli]
MISAPEPLHAGHILTPFCCGVDSMDNWLKQRAMKNQVTGASRTFVCCGSDSKVLAYYSLASSAVTTNTAPGRFRRNMPDP
IPVVVLGRLAVDQSIHGQGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLLVTLGDLV
HA

Nucleotide

• Length: 489 bp

>T186128 NZ_CP089224:1656298-1656579 [Mycobacterium ostraviense]
GTGGCTAACTCCGATGACGTCTACACGACCCGATTCACGGCCACAGGCAAGCGTGACCTCGACAAGTTGCCGCCGCGGAT
CTTCGCGGCGGTCATCGAATTCGCGTTTGGCGACTTGGCGCGCGAACCGCGCCGCGTCGGCAAGCCGCTCGGTCGCGACC
TGGCTAACCATCACAGTGCCCGACGTGGCCCCTACCGACTTCTCTACCGCATCGATGACGAGGCCCAGACGATCTGGGTC
CATCGCGTCGACCATCGTTCCGACGTTTATCGGCACCCGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10949.56 Da
• Isoelectric Point: 9.9171

>AT186128 WP_001110459.1 NZ_CP067342:848823-849116 [Escherichia coli]
MPAANSMAMKRETLNLRIRPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQTFLTRLDQAPAPN
SALRKTMQTPAPWEQEK

Nucleotide

• Length: 294 bp

>AT186128 NZ_CP089224:1656015-1656296 [Mycobacterium ostraviense]
ATGCGCGTCATACCGGTTACTCAAGTCAAGGCCAAGATCAACGAGTACGTCGACGCGGTTCGTGACACCCGCGATCAGAT
CACGATTACCAAGAACGGGGCCCCGGCCGCGGTGTTGATCGGTGTCGACGAGTGGGAATCCATTCAGGAGACCTTGCACT
GGCTGTCGCAGCCGGGCGTGGCCGAGTCGGTGCAACAGTCCGAGGCGGATATCGCCGCCGGCCGGACTTATGGCGAAGAT
GAGATCCGAGCCGAGTATCAGGTGCCTCGGCGGCCCCACTGA

Structures

Antitoxin

• AlphaFold DB: A0A7H9KCB5