Detailed information of TA system TA186120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 257522..258423
• Replicon: plasmid p027_SK3a
• Accession: NZ_CP067312
• Organism: Escherichia coli strain 027_SK3

Toxin (Protein)

• Gene name: -
• Uniprot ID: S1EZ83
• Locus tag: JJB21_RS23675
• Protein ID: WP_008786589.1
• Coordinates: 257522..257980 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: JJB21_RS23680
• Protein ID: WP_008786588.1
• Coordinates: 257977..258423 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB21_RS23630 (JJB21_23620)	253203..253490	+	288	WP_016241556.1	hypothetical protein	-
JJB21_RS23635 (JJB21_23625)	253501..253899	+	399	WP_007372292.1	hypothetical protein	-
JJB21_RS23640 (JJB21_23630)	253983..254309	+	327	WP_007372293.1	hypothetical protein	-
JJB21_RS23645 (JJB21_23635)	254672..255010	-	339	WP_007372294.1	hypothetical protein	-
JJB21_RS23650 (JJB21_23640)	255248..255691	+	444	WP_007372295.1	hypothetical protein	-
JJB21_RS23655 (JJB21_23645)	255810..256151	+	342	WP_007372296.1	hypothetical protein	-
JJB21_RS23660 (JJB21_23650)	256310..256864	+	555	WP_007372297.1	hypothetical protein	-
JJB21_RS23665 (JJB21_23655)	256914..257180	+	267	WP_007372298.1	hypothetical protein	-
JJB21_RS23670 (JJB21_23660)	257248..257478	-	231	WP_007372299.1	hypothetical protein	-
JJB21_RS23675 (JJB21_23665)	257522..257980	-	459	WP_008786589.1	RES family NAD+ phosphorylase	Toxin
JJB21_RS23680 (JJB21_23670)	257977..258423	-	447	WP_008786588.1	DUF2384 domain-containing protein	Antitoxin
JJB21_RS23685 (JJB21_23675)	258572..258889	+	318	WP_016241555.1	hypothetical protein	-
JJB21_RS23690 (JJB21_23680)	258931..259773	+	843	WP_007372303.1	hypothetical protein	-
JJB21_RS23695 (JJB21_23685)	259745..260023	+	279	WP_007372304.1	hypothetical protein	-
JJB21_RS23700 (JJB21_23690)	260083..260526	+	444	WP_007372305.1	hypothetical protein	-
JJB21_RS23705 (JJB21_23695)	260813..260977	+	165	WP_007372306.1	hypothetical protein	-
JJB21_RS23710 (JJB21_23700)	261545..262387	+	843	WP_007372307.1	HNH endonuclease signature motif containing protein	-
JJB21_RS23715 (JJB21_23705)	262809..263060	+	252	WP_024196080.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..293362	293362

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 17005.43 Da
• Isoelectric Point: 4.5730

>T186120 WP_008786589.1 NZ_CP067312:c257980-257522 [Escherichia coli]
VILYRLTKTRYLMTAWSGLGAKETGGRWNSVGTAMVYLSETASLTMLETLVHIHAPQLLDDFTLLSLDVPDDQIQTFDMS
RLPDNWASEDAPAELALYGDNWAESGSSIALRIPSALSPVEFNYLLNPAHPELFDLMATVKKIPFRFDSRLK

Nucleotide

• Length: 459 bp

>T186120 NZ_CP089222:1477185-1477763 [Mycobacterium intracellulare subsp. intracellulare]
ATGCAGCGACTTGACCTGGTAGTCGGGCCGAACGGTGCCGGCAAGTCGACCTTCATCGCGTTCACGCTGGCGCCGCTGTT
GCCCGGCAGCCCGGTCGTCAACGCCGATGAGATCGCAAGGCAGCGCTGGCCGGGGGAGGCGGCGGGGCGCGCCTACGACG
CCGCGCAAATCGCCGCCGCCACGCGCGCGAAGTTGATCGACCTGGGGCGGTCGTTCATCGCGGAGACCGTGTTTTCCCAC
CCGTCGAAGCTGGACCTCGTCCGCACCGCCCACCGAGCGGGCTTCACCGCCGTCCTGCACGCGATGCTCATCCCGGAAGA
CCTTGCCGTCGAACGTGTCCGGCACCGCGTGCGGGCCGGCGGCCACGACGTACCGGAAACCAAGGTCCGCGAGCGCCATC
GTCGCTTGTGGACACCGGTCACCGACGCCATCGCGCTCTCCGACGTCGCCACCGTCTACGACAGCGGCCGGCTGCGCGGG
CCGCGCATTGTCGCCCGCCTCAGCGGCGGCATGACGATCGGTTCACCGGACTGGCCGGCGTGGGCTCCGGAGATCTTGCG
GTCGCGCTGGCCGGACTAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.02 Da
• Isoelectric Point: 6.9812

>AT186120 WP_008786588.1 NZ_CP067312:c258423-257977 [Escherichia coli]
MTMKVFSPDVTKTNQHCLWQVAGLDNDGIALMDKINHGLDGTVAKHISEWANITPSELRKMSGIPNTTFNRSIKDRFTAD
QSERLVRIIRVIERAVELFEGDKEAAHKWLNEANRGLSWKSPAELVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT186120 NZ_CP089222:1476771-1477160 [Mycobacterium intracellulare subsp. intracellulare]
ATGGCCGACGCTCCCGATCGTGTGACCCGCGTGGCGTCCGACCTGATGGACAGCGCGGCCTCCGAAGGCGCCCGCCAGAG
CCGTTCGGCCAAGCAGCAACTCGACCACTGGGCGCGTGTCGGGCGCGCGGTGTCCAGCCAGCACACCGCCTCGCGGCGGC
GGGTCGAGGCCGCGCTGGCCGGCCAGCTGGCGACCGGCGAACTCACCGTCGAGGAAGGCGTGGTGTTCAACGCCGAGATC
TCCGCCGCCATCGAAGAAAGCCTGGTGCACGCCGACTACGGGGCGACGCTGGCCGGGCATGGCGTCACCACGGTCGCCCT
CAACGAGGACGGCGACATCGTCGAGCACCGGCCCGATGGCGCCGCCGTGGTGCTGGCGCGCGGGCGCTGA

Structures

Toxin

• AlphaFold DB: S1EZ83