Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3794277..3794931 Replicon chromosome
Accession NZ_CP067311
Organism Escherichia coli strain 027_SK3

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag JJB21_RS18360 Protein ID WP_000244777.1
Coordinates 3794524..3794931 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JJB21_RS18355 Protein ID WP_000354046.1
Coordinates 3794277..3794543 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB21_RS18330 (3789446) 3789446..3790189 + 744 WP_000951962.1 SDR family oxidoreductase -
JJB21_RS18335 (3790246) 3790246..3791679 - 1434 WP_191115604.1 6-phospho-beta-glucosidase BglA -
JJB21_RS18340 (3791724) 3791724..3792035 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
JJB21_RS18345 (3792199) 3792199..3792858 + 660 WP_000250274.1 hemolysin III family protein -
JJB21_RS18350 (3793054) 3793054..3794034 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JJB21_RS18355 (3794277) 3794277..3794543 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JJB21_RS18360 (3794524) 3794524..3794931 + 408 WP_000244777.1 protein YgfX Toxin
JJB21_RS18365 (3794971) 3794971..3795492 - 522 WP_001055874.1 flavodoxin FldB -
JJB21_RS18370 (3795604) 3795604..3796500 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JJB21_RS18375 (3796525) 3796525..3797235 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JJB21_RS18380 (3797241) 3797241..3798974 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T186117 WP_000244777.1 NZ_CP067311:3794524-3794931 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T186117 NZ_CP089221:5444184-5444645 [Mycobacterium intracellulare]
ATGGCCAAGCTTTCCGGATCCATCGATGTCCCGCTGCCGCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGATTGGCTGACCATCCACCGGGTGTGGCGCAGCGCATTGCCCGACGAGATCGAGAAGGGCTCGGTCGTCGAGTCGA
TTGTCGAGGTCAAGGGCATGCCCAACCGGATCAAATGGACGGTCGTGCGCTACAAGCCACCCGAGGGCATGACGCTCAAC
GGCGACGGAGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGATCCAGCCCAAAGGGGACGGCTCCCTCGTCAGTTTCGA
CGTGCACCTCGGTGGCCCCGCACTGTTCGGCCCCATCGGCATGATCGTCGCCGCCGCACTGCGCGGCGATATCGACCAGT
CGCTGGAGAACTTCGTGACGGTGTTCACCCGGCCCGATCCGAGCAGCAACGGGCATCGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT186117 WP_000354046.1 NZ_CP067311:3794277-3794543 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT186117 NZ_CP089221:5443992-5444180 [Mycobacterium intracellulare]
ATGGGATTCCTGGACAAGGCCAAGGATCTGTTGTGGCAGAACGCCGACAAGGTCGAGACGGCCATCGACAAGGCAGGCGA
GTTCGTCGACGAAAAGACGCAGGGCAAATACTCCGACACCATTCACCAGGTGCAGGAGCAGGCGAAGAAGGCGGCCGGCG
CCGCGGACACGGGCGACCAGCAGAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References