Detailed information of TA system TA186110

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 1937056..1937747
• Replicon: chromosome
• Accession: NZ_CP067311
• Organism: Escherichia coli strain 027_SK3

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: JJB21_RS09575
• Protein ID: WP_000854680.1
• Coordinates: 1937406..1937747 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: -
• Locus tag: JJB21_RS09570
• Protein ID: WP_062676555.1
• Coordinates: 1937056..1937385 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB21_RS09525 (1932507)	1932507..1933328	+	822	WP_160455852.1	DUF932 domain-containing protein	-
JJB21_RS09530 (1933545)	1933545..1934246	+	702	WP_021553244.1	WYL domain-containing protein	-
JJB21_RS09535 (1934287)	1934287..1934523	+	237	WP_001144029.1	protein YpjK	-
JJB21_RS09540 (1934523)	1934523..1934966	+	444	WP_021553245.1	lipoprotein YfjS	-
JJB21_RS09545 (1934989)	1934989..1935456	+	468	WP_001385283.1	protein YkfB	-
JJB21_RS09550 (1935533)	1935533..1935772	+	240	Protein_1896	DUF905 domain-containing protein	-
JJB21_RS09555 (1935870)	1935870..1936328	+	459	WP_000211838.1	antirestriction protein	-
JJB21_RS09560 (1936344)	1936344..1936820	+	477	WP_000811693.1	RadC family protein	-
JJB21_RS09565 (1936829)	1936829..1937050	+	222	WP_000691994.1	DUF987 domain-containing protein	-
JJB21_RS09570 (1937056)	1937056..1937385	+	330	WP_062676555.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
JJB21_RS09575 (1937406)	1937406..1937747	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
JJB21_RS09585 (1938319)	1938319..1939572	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
JJB21_RS09590 (1939584)	1939584..1940687	-	1104	WP_001285288.1	glutamate 5-kinase	-
JJB21_RS09595 (1940975)	1940975..1942030	+	1056	WP_000749863.1	phosphoporin PhoE	-
JJB21_RS09600 (1942069)	1942069..1942470	-	402	WP_000174677.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1910962..1954266	43304

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (11-108)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T186110 WP_000854680.1 NZ_CP067311:1937406-1937747 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T186110 NZ_CP089220:323524-323985 [Mycobacterium intracellulare]
ATGGCCAAGCTTTCCGGATCCATCGATGTCCCGCTGCCGCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGATTGGCTGACCATCCACCGGGTGTGGCGCAGCGCATTGCCCGACGAGATCGAGAAGGGCTCGGTCGTCGAGTCGA
TTGTCGAGGTCAAGGGCATGCCCAACCGGATCAAATGGACGGTCGTGCGCTACAAGCCACCCGAGGGCATGACGCTCAAC
GGCGACGGAGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGATCCAGCCCAAAGGGGACGGCTCCCTCGTCAGTTTCGA
CGTGCACCTCGGTGGCCCCGCACTGTTCGGCCCCATCGGCATGATCGTCGCCGCCGCACTGCGCGGCGATATCGACCAGT
CGCTGGAGAACTTCGTGACGGTGTTCACCCGGCCCGATCCGAGCAGCAACGGGCATCGCTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12501.10 Da
• Isoelectric Point: 6.2062

>AT186110 WP_062676555.1 NZ_CP067311:1937056-1937385 [Escherichia coli]
VENCNEQPYQGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCV
TLYHNDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 330 bp

>AT186110 NZ_CP089220:323332-323520 [Mycobacterium intracellulare]
ATGGGATTCCTGGACAAGGCCAAGGATCTGTTGTCGCAGAACGCCGACAAGGTCGAGACGGCCATCGACAAGGCAGGCGA
GTTCGTCGACGAAAAGACGCAGGGCAAATACTCCGACACCATTCACCAGGTGCAGGAGCAGGCGAAGAAGGCGGCGGGCG
CCGCGGACACGGGCGACCAGCAGAACTGA

Structures

Toxin

• AlphaFold DB: S1FHS4