Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1748839..1749457 Replicon chromosome
Accession NZ_CP067311
Organism Escherichia coli strain 027_SK3

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JJB21_RS08625 Protein ID WP_001291435.1
Coordinates 1749239..1749457 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JJB21_RS08620 Protein ID WP_000344800.1
Coordinates 1748839..1749213 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB21_RS08610 (1743928) 1743928..1745121 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JJB21_RS08615 (1745144) 1745144..1748293 + 3150 WP_137547729.1 efflux RND transporter permease AcrB -
JJB21_RS08620 (1748839) 1748839..1749213 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JJB21_RS08625 (1749239) 1749239..1749457 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JJB21_RS08630 (1749629) 1749629..1750180 + 552 WP_000102564.1 maltose O-acetyltransferase -
JJB21_RS08635 (1750296) 1750296..1750766 + 471 WP_000136192.1 YlaC family protein -
JJB21_RS08640 (1750930) 1750930..1752480 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JJB21_RS08645 (1752522) 1752522..1752875 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JJB21_RS08655 (1753254) 1753254..1753565 + 312 WP_000409911.1 MGMT family protein -
JJB21_RS08660 (1753596) 1753596..1754168 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T186109 WP_001291435.1 NZ_CP067311:1749239-1749457 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T186109 NZ_CP089218:c4727023-4726586 [Mycobacterium kansasii]
ATGGCGAAACTCTCCGGATCCATCGATGTCCCGTTGCCGCCGGAGGAGGCGTGGCTGCACGCCTCGAATCTGTCCCGCTA
CCGGGAGTGGCTGACGATCCACCGGGTCTGGCGCAGCAAGTTGCCCGAAACGCTGGAGAGGGGCGCGGTCGTCGAGTCGA
TCGTCGAGGTCAAGGGGATGCCCAACCGGATCAGATGGACGATCGTGCGCTACAAGCCGCCGGAAGGCATGACGCTCAAC
GGCGACGGAGTCGGCGGCGTCAAAGTCAAACTCATGGCCAAGGTCGCGCCCAAAGAGGAGGGCTCCGTCGTGAGCTTCGA
CGTGCACCTCGGCGGACCCGCCCTGTTCGGGCCGATCGGCATGATCGTCGCCGCCGCGCTCAGAAGCGACATCAGCGAAT
CCCTGCAGAACTTCGTCGAGGTGTTCGCACAGGCCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT186109 WP_000344800.1 NZ_CP067311:1748839-1749213 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT186109 NZ_CP089218:c4727207-4727028 [Mycobacterium kansasii]
ATGGGAATCATGGACAAGGCAAAAGACCTGCTGTCACAGAACGCCGACAAGGTCGAGACGGTGATCGACAAGGCCGGCAA
ATTCGTCGACGACAAGACGCAGGGCAAGTACTCCGACACCATTCACAAGGTGCAGGAGCAGGCCAAGAAAGTAGTCGACA
CGGGCGACCAGCAGAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References