Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 25006..25528 Replicon plasmid pABW_A19b
Accession NZ_CP067309
Organism Escherichia coli strain ABW_A19

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag JJB22_RS26130 Protein ID WP_000220560.1
Coordinates 25247..25528 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag JJB22_RS26125 Protein ID WP_000121743.1
Coordinates 25006..25257 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB22_RS26100 (JJB22_26095) 21476..21784 + 309 WP_097680207.1 hypothetical protein -
JJB22_RS26105 (JJB22_26100) 21835..22532 + 698 WP_251530579.1 IS1-like element IS1A family transposase -
JJB22_RS26110 (JJB22_26105) 22543..22989 - 447 WP_249820532.1 hypothetical protein -
JJB22_RS26115 (JJB22_26110) 23010..23849 - 840 WP_097680168.1 replication initiation protein -
JJB22_RS26120 (JJB22_26115) 24065..24220 + 156 WP_176374138.1 hypothetical protein -
JJB22_RS26125 (JJB22_26120) 25006..25257 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
JJB22_RS26130 (JJB22_26125) 25247..25528 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JJB22_RS26135 (JJB22_26130) 25674..25997 + 324 WP_001181903.1 hypothetical protein -
JJB22_RS26140 (JJB22_26135) 26042..26287 + 246 WP_000356546.1 hypothetical protein -
JJB22_RS26145 (JJB22_26140) 26277..26492 + 216 WP_001180116.1 hypothetical protein -
JJB22_RS26150 (JJB22_26145) 26585..26914 + 330 WP_000866649.1 hypothetical protein -
JJB22_RS26155 (JJB22_26150) 26992..27513 + 522 Protein_35 IS4-like element IS186B family transposase -
JJB22_RS26160 (JJB22_26155) 27687..28463 - 777 Protein_36 IS91 family transposase -
JJB22_RS26165 (JJB22_26160) 28585..28881 + 297 WP_089519066.1 hypothetical protein -
JJB22_RS26170 (JJB22_26165) 28987..29808 + 822 WP_001444560.1 DUF932 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..68410 68410
- inside IScluster/Tn - - 21835..28262 6427


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T186098 WP_000220560.1 NZ_CP067309:25247-25528 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T186098 NZ_CP089216:c6079215-6078562 [Mycobacterium kansasii]
GTGAGCACCACCATCGGACCCAACGAGCACGGCATCGAGGAAATTTTTCAAGCCCTCGACACGCTTGAAGCGTGGCGCGC
TGCGCATGCCCGTCCCCTTCAGGCCGCGACCATGGGCCTGCGTTCGCGGGTAGCCACGATGGGCTGCTCGCAGATCGAGG
TATCACAGCGGCTAGAACGCATTCCGACGATCATCGACAAACTGCGTCGCGAACCGGGCATGAACCTCGGGCGGATGGCA
GACATCGGTGGCTGTCGAGCCGTTTTGCGCGACGTCGACGAGGTGCGGCGCGTACAGTCCCGCTACGCCGGCGCCGCCGT
TACCGTCAGAACCAGGGATTACGTTGAGCAGCCAAAGTCGGACGGCTATCGCGCCGTACATGTGATCGTCCGCTACCACG
GTCGCCTGATCGAGGTTCAGCTGCGAACGCAGGTGCAACACGAATGGGCCTATACGGTGGAGAGCGTTACGTCGCGCTTC
GGGCTCGACATCAAGGCCGGTGGCGGGCCGCGGCCGGTGCATGACTGGTTCGTAGCGGTGTCAGAAGCGATGGCGCTGGA
AGAATACGGTGAGCCGGTAGCGCCGGAGCTGTTGCGGCGTGTAGATACACTGAGGGCAGCCGCCCAGCCTTACCTGCAAG
GAGTACGACGATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT186098 WP_000121743.1 NZ_CP067309:25006-25257 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT186098 NZ_CP089216:c6078565-6078311 [Mycobacterium kansasii]
ATGACTGCATCGCTGAATCACTTCTTGCTCGTCTTCGACCACAGCCGGGCCGAGCTGGTGGACCTGAAGAACTTCGGCAC
CGACGCGAAGAGTGCGATGGCGGAATACGCCCGCTTAGAGCGAGAGCACATGGGCAAGTCGGACGCGATTGAGATCGTGC
TGATCGGTTCCGATTCACTGGAAACGGTAAAGGTCACGCACGCCAACTACTTCGATGGCACCGTTGCGGTCTCAAAGTAT
TTTGCTGGCATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References