Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-DnaT |
| Location | 25006..25528 | Replicon | plasmid pABW_A19b |
| Accession | NZ_CP067309 | ||
| Organism | Escherichia coli strain ABW_A19 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | G3CAN5 |
| Locus tag | JJB22_RS26130 | Protein ID | WP_000220560.1 |
| Coordinates | 25247..25528 (+) | Length | 94 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | G3CAG1 |
| Locus tag | JJB22_RS26125 | Protein ID | WP_000121743.1 |
| Coordinates | 25006..25257 (+) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JJB22_RS26100 (JJB22_26095) | 21476..21784 | + | 309 | WP_097680207.1 | hypothetical protein | - |
| JJB22_RS26105 (JJB22_26100) | 21835..22532 | + | 698 | WP_251530579.1 | IS1-like element IS1A family transposase | - |
| JJB22_RS26110 (JJB22_26105) | 22543..22989 | - | 447 | WP_249820532.1 | hypothetical protein | - |
| JJB22_RS26115 (JJB22_26110) | 23010..23849 | - | 840 | WP_097680168.1 | replication initiation protein | - |
| JJB22_RS26120 (JJB22_26115) | 24065..24220 | + | 156 | WP_176374138.1 | hypothetical protein | - |
| JJB22_RS26125 (JJB22_26120) | 25006..25257 | + | 252 | WP_000121743.1 | plasmid stabilization protein | Antitoxin |
| JJB22_RS26130 (JJB22_26125) | 25247..25528 | + | 282 | WP_000220560.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| JJB22_RS26135 (JJB22_26130) | 25674..25997 | + | 324 | WP_001181903.1 | hypothetical protein | - |
| JJB22_RS26140 (JJB22_26135) | 26042..26287 | + | 246 | WP_000356546.1 | hypothetical protein | - |
| JJB22_RS26145 (JJB22_26140) | 26277..26492 | + | 216 | WP_001180116.1 | hypothetical protein | - |
| JJB22_RS26150 (JJB22_26145) | 26585..26914 | + | 330 | WP_000866649.1 | hypothetical protein | - |
| JJB22_RS26155 (JJB22_26150) | 26992..27513 | + | 522 | Protein_35 | IS4-like element IS186B family transposase | - |
| JJB22_RS26160 (JJB22_26155) | 27687..28463 | - | 777 | Protein_36 | IS91 family transposase | - |
| JJB22_RS26165 (JJB22_26160) | 28585..28881 | + | 297 | WP_089519066.1 | hypothetical protein | - |
| JJB22_RS26170 (JJB22_26165) | 28987..29808 | + | 822 | WP_001444560.1 | DUF932 domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | - | 1..68410 | 68410 | |
| - | inside | IScluster/Tn | - | - | 21835..28262 | 6427 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 94 a.a. Molecular weight: 11005.83 Da Isoelectric Point: 10.5938
>T186098 WP_000220560.1 NZ_CP067309:25247-25528 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR
Download Length: 282 bp
>T186098 NZ_CP089216:c6079215-6078562 [Mycobacterium kansasii]
GTGAGCACCACCATCGGACCCAACGAGCACGGCATCGAGGAAATTTTTCAAGCCCTCGACACGCTTGAAGCGTGGCGCGC
TGCGCATGCCCGTCCCCTTCAGGCCGCGACCATGGGCCTGCGTTCGCGGGTAGCCACGATGGGCTGCTCGCAGATCGAGG
TATCACAGCGGCTAGAACGCATTCCGACGATCATCGACAAACTGCGTCGCGAACCGGGCATGAACCTCGGGCGGATGGCA
GACATCGGTGGCTGTCGAGCCGTTTTGCGCGACGTCGACGAGGTGCGGCGCGTACAGTCCCGCTACGCCGGCGCCGCCGT
TACCGTCAGAACCAGGGATTACGTTGAGCAGCCAAAGTCGGACGGCTATCGCGCCGTACATGTGATCGTCCGCTACCACG
GTCGCCTGATCGAGGTTCAGCTGCGAACGCAGGTGCAACACGAATGGGCCTATACGGTGGAGAGCGTTACGTCGCGCTTC
GGGCTCGACATCAAGGCCGGTGGCGGGCCGCGGCCGGTGCATGACTGGTTCGTAGCGGTGTCAGAAGCGATGGCGCTGGA
AGAATACGGTGAGCCGGTAGCGCCGGAGCTGTTGCGGCGTGTAGATACACTGAGGGCAGCCGCCCAGCCTTACCTGCAAG
GAGTACGACGATGA
GTGAGCACCACCATCGGACCCAACGAGCACGGCATCGAGGAAATTTTTCAAGCCCTCGACACGCTTGAAGCGTGGCGCGC
TGCGCATGCCCGTCCCCTTCAGGCCGCGACCATGGGCCTGCGTTCGCGGGTAGCCACGATGGGCTGCTCGCAGATCGAGG
TATCACAGCGGCTAGAACGCATTCCGACGATCATCGACAAACTGCGTCGCGAACCGGGCATGAACCTCGGGCGGATGGCA
GACATCGGTGGCTGTCGAGCCGTTTTGCGCGACGTCGACGAGGTGCGGCGCGTACAGTCCCGCTACGCCGGCGCCGCCGT
TACCGTCAGAACCAGGGATTACGTTGAGCAGCCAAAGTCGGACGGCTATCGCGCCGTACATGTGATCGTCCGCTACCACG
GTCGCCTGATCGAGGTTCAGCTGCGAACGCAGGTGCAACACGAATGGGCCTATACGGTGGAGAGCGTTACGTCGCGCTTC
GGGCTCGACATCAAGGCCGGTGGCGGGCCGCGGCCGGTGCATGACTGGTTCGTAGCGGTGTCAGAAGCGATGGCGCTGGA
AGAATACGGTGAGCCGGTAGCGCCGGAGCTGTTGCGGCGTGTAGATACACTGAGGGCAGCCGCCCAGCCTTACCTGCAAG
GAGTACGACGATGA
Antitoxin
Download Length: 84 a.a. Molecular weight: 9194.43 Da Isoelectric Point: 4.1683
>AT186098 WP_000121743.1 NZ_CP067309:25006-25257 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL
Download Length: 252 bp
>AT186098 NZ_CP089216:c6078565-6078311 [Mycobacterium kansasii]
ATGACTGCATCGCTGAATCACTTCTTGCTCGTCTTCGACCACAGCCGGGCCGAGCTGGTGGACCTGAAGAACTTCGGCAC
CGACGCGAAGAGTGCGATGGCGGAATACGCCCGCTTAGAGCGAGAGCACATGGGCAAGTCGGACGCGATTGAGATCGTGC
TGATCGGTTCCGATTCACTGGAAACGGTAAAGGTCACGCACGCCAACTACTTCGATGGCACCGTTGCGGTCTCAAAGTAT
TTTGCTGGCATCTAA
ATGACTGCATCGCTGAATCACTTCTTGCTCGTCTTCGACCACAGCCGGGCCGAGCTGGTGGACCTGAAGAACTTCGGCAC
CGACGCGAAGAGTGCGATGGCGGAATACGCCCGCTTAGAGCGAGAGCACATGGGCAAGTCGGACGCGATTGAGATCGTGC
TGATCGGTTCCGATTCACTGGAAACGGTAAAGGTCACGCACGCCAACTACTTCGATGGCACCGTTGCGGTCTCAAAGTAT
TTTGCTGGCATCTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | G3CAN5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3S5I301 |