Detailed information of TA system TA186097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 1773..2320
• Replicon: plasmid pABW_A19b
• Accession: NZ_CP067309
• Organism: Escherichia coli strain ABW_A19

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G7QEJ9
• Locus tag: JJB22_RS26000
• Protein ID: WP_001384452.1
• Coordinates: 2042..2320 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: JJB22_RS25995
• Protein ID: WP_021548793.1
• Coordinates: 1773..2042 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB22_RS25990 (JJB22_25985)	1..858	+	858	WP_034169384.1	incFII family plasmid replication initiator RepA	-
JJB22_RS25995 (JJB22_25990)	1773..2042	+	270	WP_021548793.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
JJB22_RS26000 (JJB22_25995)	2042..2320	+	279	WP_001384452.1	type II toxin-antitoxin system toxin YacB	Toxin
JJB22_RS26005 (JJB22_26000)	2379..3242	+	864	WP_208782548.1	3'-5' exonuclease	-
JJB22_RS26010 (JJB22_26005)	3357..3647	-	291	Protein_4	IS91 family transposase	-
JJB22_RS27160	4277..4384	+	108	Protein_5	XRE family transcriptional regulator	-
JJB22_RS26015 (JJB22_26010)	4403..4666	+	264	WP_021548790.1	hypothetical protein	-
JJB22_RS26020 (JJB22_26015)	4656..4994	+	339	WP_021548789.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JJB22_RS26025 (JJB22_26020)	5057..5293	-	237	WP_208782550.1	DUF465 domain-containing protein	-
JJB22_RS27165	5597..5734	+	138	Protein_9	L-threonine dehydrogenase	-
JJB22_RS26030 (JJB22_26025)	5749..6957	-	1209	WP_208782404.1	IS4-like element ISVsa5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..68410	68410

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10762.38 Da
• Isoelectric Point: 8.4615

>T186097 WP_001384452.1 NZ_CP067309:2042-2320 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T186097 NZ_CP089216:5322240-5322662 [Mycobacterium kansasii]
GTGATCCTCGACTCGTCGGCCTTCGTCGCGCTCATCCAGGGCGAGGCTCCCTACACCGAACAGATCGCGGCAGCTCTCAC
TGGGGACCGAAGTCCGGTCATGTCAACGGCGAACGCAGCTGAATGCCTGATCGTGCTCACTAGCCGACACGGCTTCATCG
CCCGCACCGTCTTCGACCGCCTGCGATTCGAAATCAACCTCGGCTTCCAGCCGTTCAACTTCGAGCTCGCGATGGCCGCG
CATCTCGCATACCTGCGATATGGAAGGGGCCGACACCCTGCTGGCCTCAACTGCGGGGACACCATGGCCTACGCCACCGC
CAAACTCGCGCACGAACCGCTCATCGCGATCGGCAATGACTTCGCTCAAACAGACCTCGAATCCGACGGTGTCATCGGCT
ACTGGCCATCGCCCTGCGGCTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10196.52 Da
• Isoelectric Point: 8.8735

>AT186097 WP_021548793.1 NZ_CP067309:1773-2042 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSATAWRD
EMSRKAAGK

Nucleotide

• Length: 270 bp

>AT186097 NZ_CP089216:5321970-5322233 [Mycobacterium kansasii]
GTGGCATTCAACGTCAAGGATGAAGAGGTGATCCGCTTGGCCGATGAGCTCGCCGCACGTCTTCACCACCCGAGCCGCAT
CGACGCTATCCGGTATGCACTGCGCGCCCAGATCGAGATCACGCAGTCCCGCACTGCAAACCGTGCCGATGAACTCCTCG
ACGTCCTCAGAACCGAGATCTGGCCATTGCTGCACGATCGATCCCCGATCACCAAGTCCGAACGCGAACAAGCCCTCGGA
TACGACGCGGCGACGGGCGTGTGA

Structures

Toxin

• AlphaFold DB: A0A829CL36