Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 85284..86907 Replicon plasmid pABW_A19a
Accession NZ_CP067308
Organism Escherichia coli strain ABW_A19

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag JJB22_RS25210 Protein ID WP_000088046.1
Coordinates 85582..86907 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag JJB22_RS25205 Protein ID WP_024136327.1
Coordinates 85284..85577 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB22_RS25180 (JJB22_25175) 81156..82397 + 1242 WP_001053338.1 VWA domain-containing protein -
JJB22_RS27270 82488..82619 + 132 WP_000374059.1 hypothetical protein -
JJB22_RS25185 (JJB22_25180) 82960..83241 - 282 WP_000077926.1 hypothetical protein -
JJB22_RS25190 (JJB22_25185) 83291..83482 - 192 WP_000398480.1 hypothetical protein -
JJB22_RS25195 (JJB22_25190) 83574..83915 - 342 WP_001151575.1 hypothetical protein -
JJB22_RS25200 (JJB22_25195) 84288..84680 + 393 WP_000341066.1 hypothetical protein -
JJB22_RS25205 (JJB22_25200) 85284..85577 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
JJB22_RS25210 (JJB22_25205) 85582..86907 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
JJB22_RS25215 (JJB22_25210) 86968..87174 + 207 WP_000134171.1 hypothetical protein -
JJB22_RS25220 (JJB22_25215) 87276..87686 + 411 WP_000985911.1 hypothetical protein -
JJB22_RS25225 (JJB22_25220) 87699..88514 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
JJB22_RS25230 (JJB22_25225) 88768..89193 + 426 WP_001043843.1 hypothetical protein -
JJB22_RS25235 (JJB22_25230) 89647..89883 + 237 Protein_88 type I restriction-modification system subunit M N-terminal domain-containing protein -
JJB22_RS25240 (JJB22_25235) 89938..90237 + 300 WP_001572440.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid floR / sul2 / blaCTX-M-65 / fosA3 - 1..226468 226468


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T186096 WP_000088046.1 NZ_CP067308:85582-86907 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T186096 NZ_CP089216:5178743-5179126 [Mycobacterium kansasii]
GTGACGGAATTCTTCGATCGTGACGACGTCCTCACCGCCGGCGCGCTCGCCGTGGGCGCGGTGCTCGAGGTCGGCGACTT
CGGGCTGCTCGACGCCGCGGTCGCGCGACCTCAGGCCACCGTTTACGGGGTCGACGCCTACCCCGATCTCTATACCAAGG
CAGCGGCGCTGTTACAGTCGCTGGCCCGCAACCGTGCTCTGGTAGACGGCAATAAGCGAACCGCCTGGGCGTCGGCGTGG
ACGTTCTTGTATATCAACGGGATCGAGCTTGTGGCCGGGTTTGATGTAGACACCGCCGAGAGGTTCATGTACGACGTAGC
CGAGCGCGGTGAACTGACCGTCGACGACATCGCCGAAACACTGCGCCAGTTCGCGTCCGCCTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT186096 WP_024136327.1 NZ_CP067308:85284-85577 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT186096 NZ_CP089216:5178549-5178746 [Mycobacterium kansasii]
GTGCCATCGCTGAACATCGGTTTCACCGACGAAGAACTTGTTGCCGTTCGCGACGCAGCAGCCGGTGAAAACTTGTCGCT
GCGCGCGTTTGTTCACCGCGCGGCCGTCGTCGCCGCCAGCGATCGCAAGCGACGGGTCGCCGAAGCTGCGGCTCTGGTGG
CTCAGCGCTCCGCGGAGCTGAACCGCCGCCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References