Detailed information of TA system TA186095

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4706405..4707036
• Replicon: chromosome
• Accession: NZ_CP067307
• Organism: Escherichia coli strain ABW_A19

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: JJB22_RS22355
• Protein ID: WP_072686259.1
• Coordinates: 4706761..4707036 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q31VE4
• Locus tag: JJB22_RS22350
• Protein ID: WP_000593554.1
• Coordinates: 4706405..4706764 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB22_RS22325 (4702537)	4702537..4703481	+	945	WP_000947082.1	nickel ABC transporter permease subunit NikB	-
JJB22_RS22330 (4703478)	4703478..4704311	+	834	WP_072686260.1	nickel ABC transporter permease subunit NikC	-
JJB22_RS22335 (4704311)	4704311..4705075	+	765	WP_047644476.1	nickel import ATP-binding protein NikD	-
JJB22_RS22340 (4705072)	4705072..4705878	+	807	WP_032288306.1	nickel import ATP-binding protein NikE	-
JJB22_RS22345 (4705884)	4705884..4706285	+	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
JJB22_RS22350 (4706405)	4706405..4706764	-	360	WP_000593554.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JJB22_RS22355 (4706761)	4706761..4707036	-	276	WP_072686259.1	type II toxin-antitoxin system HicA family toxin	Toxin
JJB22_RS22360 (4707109)	4707109..4708233	-	1125	WP_208782522.1	ABC transporter permease	-
JJB22_RS22365 (4708233)	4708233..4710968	-	2736	WP_072686258.1	ribosome-associated ATPase/putative transporter RbbA	-
JJB22_RS22370 (4710965)	4710965..4712032	-	1068	WP_000361517.1	HlyD family efflux transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10166.87 Da
• Isoelectric Point: 10.7076

>T186095 WP_072686259.1 NZ_CP067307:c4707036-4706761 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESQVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T186095 NZ_CP089216:c5101652-5101263 [Mycobacterium kansasii]
GTGATTGTGTTGGATGCCTCGGCGGCTGTCGAGCTTGTCCTCGCGACGCCGCCTGGTGCGGCCGTCGCCCGCCGCCTGCG
CGACGAGACCGTGCATGCGCCTGCCCACATCGATGTCGAGGTGATCGGGGCGATACGTCGAGCAGTCGTGCGCCGGCTGA
TCAGCGATCATGAAGGAGTCGTCGCGGTTGCCGACTTTCTAAGCCTGCAGATTCGGCGTTGGCCTATAAAGCCATTCGCC
CAGCGCGCCTATCAACTGCGGACCACTCATATCGTGGCCGACGCAGTTTACGTCGCCCTCGCCGAGGGCTTCGGCGTACG
GCTGATCACTTGCGACGGGCGACTCGCGCAATCCCACGGCCACGATGCAGAGATCGAGCTCGTCGCCTGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13359.25 Da
• Isoelectric Point: 4.8838

>AT186095 WP_000593554.1 NZ_CP067307:c4706764-4706405 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT186095 NZ_CP089216:c5101876-5101649 [Mycobacterium kansasii]
ATGTCCACTATGGTTCAAATCCGCAACGTCCCTGACGAACTTGTCCACGAGCTGAAGGCCCGTGCAGCGGCGCAGCGAAT
GAGCCTCAGTGATTTTCTGCTGGCACGGCTCGCCGAGTTTGCGCAAGAACCGGCCCTCGACGAGGTTCTCGGCCGTCTCG
CGGCGCTGCCACGGCGGGATCTCGGCGCGAGCGCAGCTGAACTGGTTGGCGAGGCTCGATCCGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6H2GM94