Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4259814..4260655 Replicon chromosome
Accession NZ_CP067307
Organism Escherichia coli strain ABW_A19

Toxin (Protein)


Gene name yeeV Uniprot ID A0A1M0WJM1
Locus tag JJB22_RS20080 Protein ID WP_000854822.1
Coordinates 4259814..4260197 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A1M0CWL2
Locus tag JJB22_RS20085 Protein ID WP_053271974.1
Coordinates 4260287..4260655 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB22_RS20050 (4255459) 4255459..4257300 + 1842 WP_000437371.1 RNA polymerase sigma factor RpoD -
JJB22_RS20055 (4257379) 4257379..4257885 - 507 WP_000228930.1 G/U mismatch-specific DNA glycosylase -
JJB22_RS20065 (4258184) 4258184..4259029 - 846 WP_001280435.1 DUF4942 domain-containing protein -
JJB22_RS20070 (4259126) 4259126..4259323 - 198 WP_000839263.1 DUF957 domain-containing protein -
JJB22_RS20075 (4259335) 4259335..4259823 - 489 WP_001177592.1 DUF5983 family protein -
JJB22_RS20080 (4259814) 4259814..4260197 - 384 WP_000854822.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
JJB22_RS20085 (4260287) 4260287..4260655 - 369 WP_053271974.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
JJB22_RS20090 (4260735) 4260735..4260956 - 222 WP_000692312.1 DUF987 domain-containing protein -
JJB22_RS20095 (4261025) 4261025..4261501 - 477 WP_001186747.1 RadC family protein -
JJB22_RS20100 (4261516) 4261516..4262001 - 486 WP_000214317.1 antirestriction protein -
JJB22_RS20105 (4262092) 4262092..4262910 - 819 WP_001234702.1 DUF932 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / armA / msr(E) / mph(E) rfaE 4213269..4310234 96965


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14295.32 Da        Isoelectric Point: 8.2830

>T186092 WP_000854822.1 NZ_CP067307:c4260197-4259814 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHQESKRCN

Download         Length: 384 bp

>T186092 NZ_CP089216:c2423425-2423078 [Mycobacterium kansasii]
ATGTCCTGGGATGTGATCCTGCTCGAGGAGGTCGAAGCCTGGTACTTCTCGCTCGACGACGAAGCGATGGCAGCAGTCAC
CGGGGCCATCGACCTGCTCGAGCTGGAAGGACCAACGCTAGGCCGGCCGACGGTGGACAAGGTAAAGGGATCGAAGTTCC
ACAGCTTGAAGGAGCTGCGGCCGGCGGGTACCAGCATTCGGATCCTGTTCATCTTCGATCCACGACGGCAGGCGATCCTG
CTGCTTGGCGGGGACAAGGCAGGCAACTGGAAGCGCTGGTACGAGGAGAACATTCCGGTGGCCGATCAGCGATATGAGAA
CTGGCTGGCGAACGAGGGAGGTGAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13679.41 Da        Isoelectric Point: 6.4758

>AT186092 WP_053271974.1 NZ_CP067307:c4260655-4260287 [Escherichia coli]
VSDTFSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLTDRAGIRGRFSDADANHLDQAFPLLMKQLELMLTSS
ELNPHRQNTVTLYVKGLTCHADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT186092 NZ_CP089216:c2423081-2422752 [Mycobacterium kansasii]
ATGATCGTGGCACGCAACTGGCGTGAGATTCGTGCGGAGGCGGTTGCGCAGGGGCGGCTGGACCCGGCGCGCGCCGACGC
AGCACGCAAAGAAATGCACGACGCCGTCCAGGCCCAGCGCCTTGCTGACATTCGCAAGGCGCATGGACATGCCCGGCAGG
CCGACGTCGCCGCTTTGATGGGGGTGTCGCAGGCACGCGTGTCAAAGCTCGAAAGCGGCGACCTGTCGCACACGGAGCTG
GGCACGCTGCAGTCGTACGTCGCCGCCCTCGGCGGGAATCTTCGAATCGTTGCCGACTTCGGCGAGAGCAGCGTGGAACT
GACAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0WJM1

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CWL2

References