Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3941807..3942461 Replicon chromosome
Accession NZ_CP067307
Organism Escherichia coli strain ABW_A19

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag JJB22_RS18535 Protein ID WP_000244777.1
Coordinates 3941807..3942214 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JJB22_RS18540 Protein ID WP_000354046.1
Coordinates 3942195..3942461 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB22_RS18515 (3937764) 3937764..3939497 - 1734 WP_000813188.1 single-stranded-DNA-specific exonuclease RecJ -
JJB22_RS18520 (3939503) 3939503..3940213 - 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JJB22_RS18525 (3940238) 3940238..3941134 - 897 WP_000806630.1 site-specific tyrosine recombinase XerD -
JJB22_RS18530 (3941246) 3941246..3941767 + 522 WP_001055874.1 flavodoxin FldB -
JJB22_RS18535 (3941807) 3941807..3942214 - 408 WP_000244777.1 protein YgfX Toxin
JJB22_RS18540 (3942195) 3942195..3942461 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JJB22_RS18545 (3942713) 3942713..3943693 + 981 WP_021577896.1 tRNA-modifying protein YgfZ -
JJB22_RS18550 (3943770) 3943770..3944429 - 660 WP_000250270.1 hemolysin III family protein -
JJB22_RS18555 (3944593) 3944593..3944904 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
JJB22_RS18560 (3944949) 3944949..3946382 + 1434 WP_021577897.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T186089 WP_000244777.1 NZ_CP067307:c3942214-3941807 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T186089 NZ_CP089215:c6446916-6446587 [Mycolicibacterium smegmatis]
GTGCGGCGCGGCGATATCTACACCGCGGCGGCGCGAGGTGCGTACACCGGCAAGCCTCGGCCGGTCCTCATCATCCAGGA
CGACCGATTCGATGCCACCGCGTCGGTCACCGTCGTGCCGTTTACGACAAGCGACACCGACGCACCGCTTCTGCGAATTC
GAATCGAGCCAACGCCAGCCACCGGACTCTCCACAGTCAGCTCGTTGATGATCGACAAGGTGACCACAGTTCCCCGCTCC
AGCTTGACGCACCGTGTCGGCCGACTGGCGGAGACAGACATGGTCAGGACCGACCGCGCACTCCTGGTTTTTCTGGGAAT
CGCCGGGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT186089 WP_000354046.1 NZ_CP067307:c3942461-3942195 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT186089 NZ_CP089215:c6447128-6446919 [Mycolicibacterium smegmatis]
ATGACTCCCGCGCGTGACCGAGTACGCCGACATCGCGAGCGGCTGCGGCGACAAGGTCTGCGGCCGGTGCAGATCTGGGT
CCCCGACGTGAATGCACCCGAATTTCGCCGGGAGGCGCACCGGCAGTCTGAGTTGGTCGCCGCCGGCGAGCATGAGGCCG
AAGATCAAGCTTTCGTCGACGCGATCTCCGTCGACTGGGACGACGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References