Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3796433..3797016 Replicon chromosome
Accession NZ_CP067307
Organism Escherichia coli strain ABW_A19

Toxin (Protein)


Gene name mazF Uniprot ID U9Y5H3
Locus tag JJB22_RS17895 Protein ID WP_000254735.1
Coordinates 3796433..3796768 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A246NW33
Locus tag JJB22_RS17900 Protein ID WP_021577864.1
Coordinates 3796768..3797016 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB22_RS17880 (3792319) 3792319..3793617 - 1299 WP_000036723.1 phosphopyruvate hydratase -
JJB22_RS17885 (3793705) 3793705..3795342 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
JJB22_RS17890 (3795570) 3795570..3796361 - 792 WP_021577863.1 nucleoside triphosphate pyrophosphohydrolase -
JJB22_RS17895 (3796433) 3796433..3796768 - 336 WP_000254735.1 endoribonuclease MazF Toxin
JJB22_RS17900 (3796768) 3796768..3797016 - 249 WP_021577864.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
JJB22_RS17905 (3797094) 3797094..3799328 - 2235 WP_000226815.1 GTP pyrophosphokinase -
JJB22_RS17910 (3799376) 3799376..3800677 - 1302 WP_021577865.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12070.03 Da        Isoelectric Point: 8.2605

>T186088 WP_000254735.1 NZ_CP067307:c3796768-3796433 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRAKGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T186088 NZ_CP089215:5245466-5245909 [Mycolicibacterium smegmatis]
ATGGCCAAACTTTCCGTCTCTGTCGAAGTTCCTTTGCCGCCGGAGCAGGCGTGGGAGTATGCCTCCGATCTGTCCCGGTA
CCACGAGTGGCTCAGCATTCACCGGGCCTGGCGCTCGAAGTTGCCCGAGACCCTGGAGAAGGGCACGGTGATCGAGTCGA
TCGTCGAGGTCAAGGGCATGCTCAACCGGGTCAGGTGGACGCTGGTCCACTACAAGCCCCCGCAGGCGCTCACACTCAAC
GGTGACGGCCGCGGTGGGGTCAAGGTCAAGCTGATCGGCAAGATCAAGCCCACCGACAACGGCGCGACGGTGGGCTTCGA
CCTGCACCTCGGCGGCCCGGCCCTGTTCGGGCCCATCGGCATGGTGGTGGCCGCGGCGCTCAAGAGCGACATCCAGGAGT
CGCTCAACCGCTTCAAGCAGCTCTACGCCCCGTCCGCGACCTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9369.76 Da        Isoelectric Point: 4.4665

>AT186088 WP_021577864.1 NZ_CP067307:c3797016-3796768 [Escherichia coli]
MIHSSIKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT186088 NZ_CP089215:5245268-5245456 [Mycolicibacterium smegmatis]
ATGGGATTCCTGGACAAGGCCAAAGACCTCTTGTCGCAGAACGCCGACAAGGTGGACACCGTCATTGACAAGGCCGGCGA
CATGATCGACGAGAAGACGCAGGGCAAGTACGCCCAGCACGTCGACAAGGCGCAGGACGCCGCGAAGAACGCCATCCGCA
AAGACGACCCTCAAGAACCACAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9Y5H3

Antitoxin

Source ID Structure
AlphaFold DB A0A246NW33

References