Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3485364..3485989 Replicon chromosome
Accession NZ_CP067307
Organism Escherichia coli strain ABW_A19

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag JJB22_RS16385 Protein ID WP_000911329.1
Coordinates 3485364..3485762 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag JJB22_RS16390 Protein ID WP_000450524.1
Coordinates 3485762..3485989 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB22_RS16365 (3481271) 3481271..3481471 + 201 WP_000383836.1 YpfN family protein -
JJB22_RS16370 (3481552) 3481552..3482250 - 699 WP_000679812.1 esterase -
JJB22_RS16375 (3482324) 3482324..3484339 - 2016 WP_072686086.1 tRNA cytosine(34) acetyltransferase TmcA -
JJB22_RS16380 (3484354) 3484354..3485217 - 864 WP_001267498.1 neutral zinc metallopeptidase -
JJB22_RS16385 (3485364) 3485364..3485762 - 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
JJB22_RS16390 (3485762) 3485762..3485989 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
JJB22_RS16395 (3486144) 3486144..3486857 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
JJB22_RS16400 (3487070) 3487070..3488104 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
JJB22_RS16405 (3488121) 3488121..3488999 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
JJB22_RS16410 (3489145) 3489145..3489717 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
JJB22_RS16415 (3489717) 3489717..3490187 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T186087 WP_000911329.1 NZ_CP067307:c3485762-3485364 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T186087 NZ_CP089215:3289310-3289741 [Mycolicibacterium smegmatis]
ATGTTCCTGCTAGACGTGAACGTCGTACTTGCGGCATACCGCGCAGACCACCCAGATCACGCCACCGTGCGTCCGTGGTT
CGATCGCCTGCTCGATGGCGACGGACCGTTCACCGTCCCGGTACAGGTGTGGGCGTCGTTCCTGCGATTGGTAACCAATC
GGCGTATCTTCCCAGTCCCCACACCCCGTGACGATGCGTTCGCGTTCATCGAAGCCGTCAACGGCCAACCGCACCACGTG
CCGGTAAGCGCGGGCCCGCGGCACCTTGCGCTGCTGCACCAACTTTGCGACGAAGGCGATGCCTCCGGCGACCTGATCCC
CGATGCAGTGCTCGCGGCCATCGCCCACGAACATCATTGCGACGTGGCGACTCTCGATCGGGACTTCGCCCGATTCACCA
CGGTCCGTCATCTGAGGCCGAGTCTGGACTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT186087 WP_000450524.1 NZ_CP067307:c3485989-3485762 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT186087 NZ_CP089215:3289044-3289301 [Mycolicibacterium smegmatis]
GTGCGCACCACGATCGCGATCGCCGATGAATTACTGGCCGCGGCCAAGCGCCGAGCCCGCCAGCGTGGCCAGTCATTGGG
CTCGGTGATCGAGGATGCTCTGCGCCGCGAACTGGCTGCCGATCAGGAAGCGGCGCCACGTCCAGAGGTTCCGGTATTCA
CCGACGGCACCGGGCCGCGTGCCGGTATCGACCTACGGTCGGCCCGCGCACTTCACGAAGTCCTCGACGAAGGCCTCGAC
CTCAACGCGCTCAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References