Detailed information of TA system TA186075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2307641..2308279
• Replicon: chromosome
• Accession: NZ_CP067307
• Organism: Escherichia coli strain ABW_A19

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A246NT23
• Locus tag: JJB22_RS10695
• Protein ID: WP_021577430.1
• Coordinates: 2307641..2307817 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JJB22_RS10700
• Protein ID: WP_001270286.1
• Coordinates: 2307863..2308279 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB22_RS10675 (2303260)	2303260..2304435	-	1176	WP_021577427.1	BenE family transporter YdcO	-
JJB22_RS10680 (2304527)	2304527..2305063	+	537	WP_021577428.1	DNA-binding transcriptional regulator SutR	-
JJB22_RS10685 (2305136)	2305136..2307097	+	1962	WP_021577429.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JJB22_RS10690 (2307189)	2307189..2307419	-	231	WP_000494244.1	YncJ family protein	-
JJB22_RS10695 (2307641)	2307641..2307817	+	177	WP_021577430.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JJB22_RS10700 (2307863)	2307863..2308279	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JJB22_RS10705 (2308358)	2308358..2309764	+	1407	WP_000760665.1	PLP-dependent aminotransferase family protein	-
JJB22_RS10710 (2310009)	2310009..2311154	+	1146	WP_021577431.1	ABC transporter substrate-binding protein	-
JJB22_RS10715 (2311172)	2311172..2312185	+	1014	WP_021577432.1	ABC transporter ATP-binding protein	-
JJB22_RS10720 (2312186)	2312186..2313127	+	942	WP_021577433.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2302072..2303220	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6692.77 Da
• Isoelectric Point: 10.9223

>T186075 WP_021577430.1 NZ_CP067307:2307641-2307817 [Escherichia coli]
VKQSEFRRWLESQGVDVANVSNHLKLGFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T186075 NZ_CP089211:1610816-1611064 [Sinomicrobium kalidii]
ATGGAAACGGTAAACTACACAGACTTTCGCGCTAACCTGAAGCATTGGTTCGACAAGGTAATCAATGATGTAAGCGATAT
TGTCATTAAGCGAAAAGGAGGAAAAGACCTTGTTCTGATTTCCCTGGATGAATACAATTCATTAAAGGAGACCACCTACC
TGCTGACCGGGAAAAACAGGGATGTTTTATTGAAGTCTATTAAGGAGCTGGAAGGCGATAAAGGTGTTCAAAAAGACCTG
ATTGAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT186075 WP_001270286.1 NZ_CP067307:2307863-2308279 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT186075 NZ_CP089211:1611064-1611324 [Sinomicrobium kalidii]
ATGAGGTTAATTTGGTCTACAACTTCCTCGAATGATTATCTGTATTGCCAAAAAGTAGATAAAAAGATCGTCAAGCGCAT
TAATGAGTTGATAAAAAACTGTATGCGCACACCCTTTGAGGGCATAGGAAAACCCGAGGCCCTGAAAGGTGGTTTACAAG
GATATTGGTCGAGACGGATAACATCAGAACATCGGCTGGTTTATAAATACGAGGATGAAGAATTGTGGATAGCGGCCTGC
CGTTATCACTATGGGAAGTGA

Structures

Toxin

• AlphaFold DB: A0A246NT23