Detailed information of TA system TA186070

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1867666..1868501
• Replicon: chromosome
• Accession: NZ_CP067307
• Organism: Escherichia coli strain ABW_A19

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: JJB22_RS08500
• Protein ID: WP_000854768.1
• Coordinates: 1868124..1868501 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JJB22_RS08495
• Protein ID: WP_024179191.1
• Coordinates: 1867666..1868034 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB22_RS08465 (1863365)	1863365..1864435	+	1071	WP_086624232.1	patatin-like phospholipase family protein	-
JJB22_RS08470 (1864775)	1864775..1865593	+	819	WP_080030209.1	DUF932 domain-containing protein	-
JJB22_RS08475 (1865686)	1865686..1866171	+	486	WP_000206651.1	antirestriction protein	-
JJB22_RS08480 (1866187)	1866187..1866663	+	477	WP_001470113.1	RadC family protein	-
JJB22_RS08485 (1866732)	1866732..1866953	+	222	WP_000692290.1	DUF987 domain-containing protein	-
JJB22_RS08490 (1866972)	1866972..1867616	+	645	WP_000086766.1	hypothetical protein	-
JJB22_RS08495 (1867666)	1867666..1868034	+	369	WP_024179191.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JJB22_RS08500 (1868124)	1868124..1868501	+	378	WP_000854768.1	TA system toxin CbtA family protein	Toxin
JJB22_RS08505 (1868498)	1868498..1868986	+	489	WP_000761708.1	DUF5983 family protein	-
JJB22_RS08510 (1868998)	1868998..1869195	+	198	WP_000839234.1	DUF957 domain-containing protein	-
JJB22_RS08515 (1869292)	1869292..1870134	+	843	WP_072693077.1	DUF4942 domain-containing protein	-
JJB22_RS08525 (1870605)	1870605..1871543	+	939	WP_000351317.1	glyoxylate/hydroxypyruvate reductase GhrA	-
JJB22_RS08530 (1871598)	1871598..1872335	+	738	WP_000283667.1	zinc-binding phosphatase	-
JJB22_RS08535 (1872359)	1872359..1872913	+	555	WP_001001921.1	molecular chaperone YcdY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14148.25 Da
• Isoelectric Point: 7.7294

>T186070 WP_000854768.1 NZ_CP067307:1868124-1868501 [Escherichia coli]
MKTLPDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
GACTCSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T186070 NZ_CP089202:c42054-41746 [Vibrio splendidus]
ATGACGCCTTTTCAGCTCACGAACAAGGCAAAGTCCGATCTAAAAGACATTGCCTTGTTTACTGAGCGTCGCTGGGGTCG
CAAGCAACGAAATGTCTATCTCAGACAGTTCGATACCACATTAGGGCGCTTAGCGGAGAATCCGGAGCTAGGAAAAACCT
GTGATGAAATCAGGCCAGGTTACCGAAAATTCCCACAGACCAGTCACGTCATTTTCTACCAGAAAATGAATGACCAACCT
CTCCTAATCATCCGAATTTTACATAAAAGTATGGATGTGAACTCCATCTTAATCTCGTTAACTATTTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13734.44 Da
• Isoelectric Point: 5.4970

>AT186070 WP_024179191.1 NZ_CP067307:1867666-1868034 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT186070 NZ_CP089202:c42293-42051 [Vibrio splendidus]
ATGGCGAGAAATACAAGCATCACCTTGGGTGAACACTTCGATGGGTTTATTGCGAGCCAGATCCAAAGCGGTCGTTATGG
CTCGGCCAGCGAGGTGATCCGCTCGGCGCTGCGTTTACTCGAAACCCAAGAGACGAAGATGAATACGTTACGACAGCTAC
TCGTGGAAGGTGAAAACAGTGGGATCGCGGATTACGACCTCGATACTTTCATTAATGAACTCGACAGTGAAGAAAGAAAA
TGA