Detailed information of TA system TA186069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1580882..1581587
• Replicon: chromosome
• Accession: NZ_CP067307
• Organism: Escherichia coli strain ABW_A19

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A246P0D5
• Locus tag: JJB22_RS07215
• Protein ID: WP_000539522.1
• Coordinates: 1581201..1581587 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JJB22_RS07210
• Protein ID: WP_001280945.1
• Coordinates: 1580882..1581211 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB22_RS07195 (1575929)	1575929..1578277	+	2349	Protein_1408	EAL domain-containing protein	-
JJB22_RS07200 (1578285)	1578285..1579613	+	1329	WP_000086864.1	GGDEF domain-containing protein	-
JJB22_RS07205 (1579766)	1579766..1580746	-	981	WP_000019440.1	IS5-like element ISKpn26 family transposase	-
JJB22_RS07210 (1580882)	1580882..1581211	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
JJB22_RS07215 (1581201)	1581201..1581587	-	387	WP_000539522.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JJB22_RS07220 (1581813)	1581813..1583138	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
JJB22_RS07225 (1583351)	1583351..1583734	+	384	WP_000497137.1	biofilm formation regulator BssR	-
JJB22_RS07230 (1583845)	1583845..1584960	+	1116	WP_021577174.1	aldose sugar dehydrogenase YliI	-
JJB22_RS07235 (1584957)	1584957..1585583	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1579766..1580746	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14292.51 Da
• Isoelectric Point: 10.2120

>T186069 WP_000539522.1 NZ_CP067307:c1581587-1581201 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIKDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T186069 NZ_CP089165:2427727-2427915 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT186069 WP_001280945.1 NZ_CP067307:c1581211-1580882 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT186069 NZ_CP089165:2427438-2427698 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A246P0D5