Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 696113..696663 Replicon chromosome
Accession NZ_CP067307
Organism Escherichia coli strain ABW_A19

Toxin (Protein)


Gene name ccdB Uniprot ID Q32K46
Locus tag JJB22_RS03200 Protein ID WP_001160974.1
Coordinates 696349..696663 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag JJB22_RS03195 Protein ID WP_000125566.1
Coordinates 696113..696346 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB22_RS03170 (691184) 691184..691471 + 288 WP_000203741.1 ferredoxin-like protein FixX -
JJB22_RS03175 (691529) 691529..692860 + 1332 WP_001183198.1 MFS transporter -
JJB22_RS03180 (692971) 692971..693501 + 531 WP_000600729.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
JJB22_RS03185 (693494) 693494..695356 + 1863 WP_000377191.1 glutathione-regulated potassium-efflux system protein KefC -
JJB22_RS03190 (695548) 695548..696027 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
JJB22_RS03195 (696113) 696113..696346 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
JJB22_RS03200 (696349) 696349..696663 + 315 WP_001160974.1 CcdB family protein Toxin
JJB22_RS03205 (696660) 696660..697508 - 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
JJB22_RS03210 (697515) 697515..697892 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
JJB22_RS03215 (697895) 697895..698716 - 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
JJB22_RS03220 (698713) 698713..699702 - 990 WP_000241272.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
JJB22_RS03225 (699702) 699702..700988 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11737.84 Da        Isoelectric Point: 9.8517

>T186066 WP_001160974.1 NZ_CP067307:696349-696663 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T186066 NZ_CP089165:1176761-1176970 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT186066 WP_000125566.1 NZ_CP067307:696113-696346 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT186066 NZ_CP089165:1176456-1176749 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LRR3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References