Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 286304..286801 Replicon chromosome
Accession NZ_CP067307
Organism Escherichia coli strain ABW_A19

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JJB22_RS01340 Protein ID WP_001173343.1
Coordinates 286628..286801 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JJB22_RS01335 Protein ID WP_000398619.1
Coordinates 286304..286600 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB22_RS01315 (283035) 283035..283754 - 720 WP_000611288.1 two-component system response regulator DcuR -
JJB22_RS01320 (283751) 283751..285382 - 1632 WP_001216477.1 sensor histidine kinase -
JJB22_RS01325 (285563) 285563..285793 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
JJB22_RS01330 (285805) 285805..286077 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
JJB22_RS01335 (286304) 286304..286600 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JJB22_RS01340 (286628) 286628..286801 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JJB22_RS01345 (286920) 286920..288437 - 1518 WP_001295074.1 lysine--tRNA ligase -
JJB22_RS01350 (288674) 288674..290131 - 1458 WP_000856835.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T186063 WP_001173343.1 NZ_CP067307:286628-286801 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T186063 NZ_CP089162:2631238-2631504 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT186063 WP_000398619.1 NZ_CP067307:286304-286600 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT186063 NZ_CP089162:2630987-2631238 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGTTATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References