Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 2607210..2608045 | Replicon | chromosome |
| Accession | NZ_CP067299 | ||
| Organism | Escherichia coli strain ABW_A33 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | A0A1X9TM58 |
| Locus tag | JJB24_RS12695 | Protein ID | WP_000854761.1 |
| Coordinates | 2607668..2608045 (+) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | S1NM52 |
| Locus tag | JJB24_RS12690 | Protein ID | WP_001295723.1 |
| Coordinates | 2607210..2607578 (+) | Length | 123 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JJB24_RS12665 (2602603) | 2602603..2603148 | - | 546 | WP_000973197.1 | bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase | - |
| JJB24_RS23810 (2603300) | 2603300..2603473 | - | 174 | Protein_2504 | cobalamin biosynthesis protein | - |
| JJB24_RS23815 (2603452) | 2603452..2603640 | - | 189 | Protein_2505 | IS4 family transposase | - |
| JJB24_RS12670 (2604201) | 2604201..2604446 | + | 246 | Protein_2506 | transposase | - |
| JJB24_RS12675 (2604614) | 2604614..2605183 | - | 570 | WP_000221554.1 | inovirus Gp2 family protein | - |
| JJB24_RS23820 (2605382) | 2605382..2605582 | + | 201 | WP_001297219.1 | hypothetical protein | - |
| JJB24_RS12680 (2605928) | 2605928..2606053 | - | 126 | Protein_2509 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| JJB24_RS12685 (2606826) | 2606826..2607047 | + | 222 | WP_000692345.1 | DUF987 domain-containing protein | - |
| JJB24_RS12690 (2607210) | 2607210..2607578 | + | 369 | WP_001295723.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| JJB24_RS12695 (2607668) | 2607668..2608045 | + | 378 | WP_000854761.1 | TA system toxin CbtA family protein | Toxin |
| JJB24_RS12700 (2608042) | 2608042..2608190 | + | 149 | Protein_2513 | DUF5983 family protein | - |
| JJB24_RS12705 (2608290) | 2608290..2608370 | + | 81 | Protein_2514 | hypothetical protein | - |
| JJB24_RS12710 (2608801) | 2608801..2610340 | - | 1540 | Protein_2515 | IS66-like element ISEc22 family transposase | - |
| JJB24_RS12715 (2610389) | 2610389..2610736 | - | 348 | WP_000612626.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| JJB24_RS12720 (2610733) | 2610733..2611137 | - | 405 | WP_000839179.1 | transposase | - |
| JJB24_RS23825 (2611218) | 2611218..2611442 | + | 225 | Protein_2518 | transposase | - |
| JJB24_RS12725 (2611631) | 2611631..2612020 | + | 390 | WP_001445118.1 | IS110 family transposase | - |
| JJB24_RS23830 (2612291) | 2612291..2612656 | + | 366 | WP_001282181.1 | EutP/PduV family microcompartment system protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 2573399..2608143 | 34744 | |
| - | inside | IScluster/Tn | - | - | 2604201..2611137 | 6936 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 14205.25 Da Isoelectric Point: 7.3249
>T186016 WP_000854761.1 NZ_CP067299:2607668-2608045 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR
Download Length: 378 bp
>T186016 NZ_CP089148:c2187911-2187735 [Bacillus subtilis]
GTGCTTGAGAAAGTGGGTATCATAGTTGCTTTCCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGGTAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA
GTGCTTGAGAAAGTGGGTATCATAGTTGCTTTCCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGGTAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA
Antitoxin
Download Length: 123 a.a. Molecular weight: 13592.40 Da Isoelectric Point: 6.6255
>AT186016 WP_001295723.1 NZ_CP067299:2607210-2607578 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK
Download Length: 369 bp
>AT186016 NZ_CP089148:2187852-2187952 [Bacillus subtilis]
GATTGTAAGAACCGTTAAAGATATGAGGAAAGCAACTATGATACCCACTTTCTCAAGCACTATGTACACCTCCTTTCCTA
TGACTCTATTATAACATAATT
GATTGTAAGAACCGTTAAAGATATGAGGAAAGCAACTATGATACCCACTTTCTCAAGCACTATGTACACCTCCTTTCCTA
TGACTCTATTATAACATAATT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1X9TM58 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | S1NM52 |