Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1050793..1051411 Replicon chromosome
Accession NZ_CP067299
Organism Escherichia coli strain ABW_A33

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JJB24_RS04985 Protein ID WP_001291435.1
Coordinates 1050793..1051011 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JJB24_RS04990 Protein ID WP_000344800.1
Coordinates 1051037..1051411 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB24_RS04950 (1046082) 1046082..1046654 + 573 WP_000779831.1 YbaY family lipoprotein -
JJB24_RS04955 (1046685) 1046685..1046996 - 312 WP_000409911.1 MGMT family protein -
JJB24_RS04965 (1047375) 1047375..1047728 + 354 WP_000878140.1 DUF1428 domain-containing protein -
JJB24_RS04970 (1047770) 1047770..1049320 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JJB24_RS04975 (1049484) 1049484..1049954 - 471 WP_000136192.1 YlaC family protein -
JJB24_RS04980 (1050070) 1050070..1050621 - 552 WP_000102564.1 maltose O-acetyltransferase -
JJB24_RS04985 (1050793) 1050793..1051011 - 219 WP_001291435.1 HHA domain-containing protein Toxin
JJB24_RS04990 (1051037) 1051037..1051411 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JJB24_RS04995 (1051957) 1051957..1055106 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JJB24_RS05000 (1055129) 1055129..1056322 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T186004 WP_001291435.1 NZ_CP067299:c1051011-1050793 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T186004 NZ_CP089142:2064658-2064761 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT186004 WP_000344800.1 NZ_CP067299:c1051411-1051037 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT186004 NZ_CP089142:c2064799-2064528 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTAGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References