186001

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 543108..543684 Replicon chromosome
Accession NZ_CP067299
Organism Escherichia coli strain ABW_A33

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag JJB24_RS02585 Protein ID WP_001297643.1
Coordinates 543108..543395 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag JJB24_RS02590 Protein ID WP_000063156.1
Coordinates 543382..543684 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB24_RS02565 (539361) 539361..540641 - 1281 WP_001037380.1 DUF445 domain-containing protein -
JJB24_RS02570 (540682) 540682..541914 - 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
JJB24_RS02575 (542060) 542060..542155 - 96 Protein_509 dienelactone hydrolase family protein -
JJB24_RS02580 (542212) 542212..542909 + 698 Protein_510 IS1 family transposase -
JJB24_RS02585 (543108) 543108..543395 + 288 WP_001297643.1 BrnT family toxin Toxin
JJB24_RS02590 (543382) 543382..543684 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
JJB24_RS02595 (543718) 543718..544674 - 957 WP_001297640.1 GTPase -
JJB24_RS02600 (544685) 544685..544888 - 204 WP_000467859.1 YbdD/YjiX family protein -
JJB24_RS02605 (544938) 544938..547088 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T186001 WP_001297643.1 NZ_CP067299:543108-543395 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T186001 NZ_CP089142:1853931-1854182 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT186001 WP_000063156.1 NZ_CP067299:543382-543684 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT186001 NZ_CP089142:1854179-1854433 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References