Detailed information of TA system TA185998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 268605..269506
• Replicon: plasmid pABW_S38a
• Accession: NZ_CP067266
• Organism: Escherichia coli strain ABW_S38

Toxin (Protein)

• Gene name: -
• Uniprot ID: S1EZ83
• Locus tag: JJB13_RS23895
• Protein ID: WP_008786589.1
• Coordinates: 268605..269063 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: JJB13_RS23900
• Protein ID: WP_008786588.1
• Coordinates: 269060..269506 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB13_RS23855 (JJB13_23850)	264113..265081	-	969	Protein_311	type II restriction endonuclease	-
JJB13_RS23860 (JJB13_23855)	265133..265837	-	705	WP_001067848.1	IS6-like element IS26 family transposase	-
JJB13_RS23865 (JJB13_23860)	265884..266093	-	210	WP_251532898.1	hypothetical protein	-
JJB13_RS23870 (JJB13_23865)	266331..266774	+	444	WP_007372295.1	hypothetical protein	-
JJB13_RS23875 (JJB13_23870)	266893..267234	+	342	WP_007372296.1	hypothetical protein	-
JJB13_RS23880 (JJB13_23875)	267393..267947	+	555	WP_007372297.1	hypothetical protein	-
JJB13_RS23885 (JJB13_23880)	267997..268263	+	267	WP_007372298.1	hypothetical protein	-
JJB13_RS23890 (JJB13_23885)	268331..268561	-	231	WP_007372299.1	hypothetical protein	-
JJB13_RS23895 (JJB13_23890)	268605..269063	-	459	WP_008786589.1	RES family NAD+ phosphorylase	Toxin
JJB13_RS23900 (JJB13_23895)	269060..269506	-	447	WP_008786588.1	DUF2384 domain-containing protein	Antitoxin
JJB13_RS23905 (JJB13_23900)	269655..269972	+	318	WP_016241555.1	hypothetical protein	-
JJB13_RS23910 (JJB13_23905)	270014..270856	+	843	WP_007372303.1	hypothetical protein	-
JJB13_RS23915 (JJB13_23910)	270828..271106	+	279	WP_007372304.1	hypothetical protein	-
JJB13_RS23920 (JJB13_23915)	271166..271609	+	444	WP_007372305.1	hypothetical protein	-
JJB13_RS23925 (JJB13_23920)	271896..272060	+	165	WP_007372306.1	hypothetical protein	-
JJB13_RS23930 (JJB13_23925)	272628..273470	+	843	WP_007372307.1	HNH endonuclease signature motif containing protein	-
JJB13_RS23935 (JJB13_23930)	273892..274143	+	252	WP_024196080.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..303836	303836

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 17005.43 Da
• Isoelectric Point: 4.5730

>T185998 WP_008786589.1 NZ_CP067266:c269063-268605 [Escherichia coli]
VILYRLTKTRYLMTAWSGLGAKETGGRWNSVGTAMVYLSETASLTMLETLVHIHAPQLLDDFTLLSLDVPDDQIQTFDMS
RLPDNWASEDAPAELALYGDNWAESGSSIALRIPSALSPVEFNYLLNPAHPELFDLMATVKKIPFRFDSRLK

Nucleotide

• Length: 459 bp

>T185998 NZ_CP089142:805129-805425 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGATTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.02 Da
• Isoelectric Point: 6.9812

>AT185998 WP_008786588.1 NZ_CP067266:c269506-269060 [Escherichia coli]
MTMKVFSPDVTKTNQHCLWQVAGLDNDGIALMDKINHGLDGTVAKHISEWANITPSELRKMSGIPNTTFNRSIKDRFTAD
QSERLVRIIRVIERAVELFEGDKEAAHKWLNEANRGLSWKSPAELVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT185998 NZ_CP089142:805427-805822 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: S1EZ83