Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3810760..3811414 Replicon chromosome
Accession NZ_CP067265
Organism Escherichia coli strain ABW_S38

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag JJB13_RS18470 Protein ID WP_000244777.1
Coordinates 3811007..3811414 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JJB13_RS18465 Protein ID WP_000354046.1
Coordinates 3810760..3811026 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB13_RS18440 (3805929) 3805929..3806672 + 744 WP_000951962.1 SDR family oxidoreductase -
JJB13_RS18445 (3806729) 3806729..3808162 - 1434 WP_191115604.1 6-phospho-beta-glucosidase BglA -
JJB13_RS18450 (3808207) 3808207..3808518 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
JJB13_RS18455 (3808682) 3808682..3809341 + 660 WP_000250274.1 hemolysin III family protein -
JJB13_RS18460 (3809537) 3809537..3810517 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JJB13_RS18465 (3810760) 3810760..3811026 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JJB13_RS18470 (3811007) 3811007..3811414 + 408 WP_000244777.1 protein YgfX Toxin
JJB13_RS18475 (3811454) 3811454..3811975 - 522 WP_001055874.1 flavodoxin FldB -
JJB13_RS18480 (3812087) 3812087..3812983 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JJB13_RS18485 (3813008) 3813008..3813718 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JJB13_RS18490 (3813724) 3813724..3815457 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T185995 WP_000244777.1 NZ_CP067265:3811007-3811414 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T185995 NZ_CP089142:c641086-640622 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT185995 WP_000354046.1 NZ_CP067265:3810760-3811026 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT185995 NZ_CP089142:c641421-641086 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References