Detailed information of TA system TA185988

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 1937153..1937844
• Replicon: chromosome
• Accession: NZ_CP067265
• Organism: Escherichia coli strain ABW_S38

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: JJB13_RS09575
• Protein ID: WP_000854680.1
• Coordinates: 1937503..1937844 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: -
• Locus tag: JJB13_RS09570
• Protein ID: WP_062676555.1
• Coordinates: 1937153..1937482 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB13_RS09525 (1932604)	1932604..1933425	+	822	WP_160455852.1	DUF932 domain-containing protein	-
JJB13_RS09530 (1933642)	1933642..1934343	+	702	WP_021553244.1	WYL domain-containing protein	-
JJB13_RS09535 (1934384)	1934384..1934620	+	237	WP_001144029.1	protein YpjK	-
JJB13_RS09540 (1934620)	1934620..1935063	+	444	WP_021553245.1	lipoprotein YfjS	-
JJB13_RS09545 (1935086)	1935086..1935553	+	468	WP_001385283.1	protein YkfB	-
JJB13_RS09550 (1935630)	1935630..1935869	+	240	Protein_1896	DUF905 domain-containing protein	-
JJB13_RS09555 (1935967)	1935967..1936425	+	459	WP_000211838.1	antirestriction protein	-
JJB13_RS09560 (1936441)	1936441..1936917	+	477	WP_000811693.1	RadC family protein	-
JJB13_RS09565 (1936926)	1936926..1937147	+	222	WP_000691994.1	DUF987 domain-containing protein	-
JJB13_RS09570 (1937153)	1937153..1937482	+	330	WP_062676555.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
JJB13_RS09575 (1937503)	1937503..1937844	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
JJB13_RS09585 (1938416)	1938416..1939669	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
JJB13_RS09590 (1939681)	1939681..1940784	-	1104	WP_001285288.1	glutamate 5-kinase	-
JJB13_RS09595 (1941072)	1941072..1942127	+	1056	WP_000749863.1	phosphoporin PhoE	-
JJB13_RS09600 (1942166)	1942166..1942567	-	402	WP_000174677.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1911058..1954363	43305

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (11-108)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T185988 WP_000854680.1 NZ_CP067265:1937503-1937844 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T185988 NZ_CP089135:c113618-113313 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12501.10 Da
• Isoelectric Point: 6.2062

>AT185988 WP_062676555.1 NZ_CP067265:1937153-1937482 [Escherichia coli]
VENCNEQPYQGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCV
TLYHNDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 330 bp

>AT185988 NZ_CP089135:c113838-113620 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: S1FHS4