Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1748935..1749553 Replicon chromosome
Accession NZ_CP067265
Organism Escherichia coli strain ABW_S38

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JJB13_RS08625 Protein ID WP_001291435.1
Coordinates 1749335..1749553 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JJB13_RS08620 Protein ID WP_000344800.1
Coordinates 1748935..1749309 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB13_RS08610 (1744024) 1744024..1745217 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JJB13_RS08615 (1745240) 1745240..1748389 + 3150 WP_137547729.1 efflux RND transporter permease AcrB -
JJB13_RS08620 (1748935) 1748935..1749309 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JJB13_RS08625 (1749335) 1749335..1749553 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JJB13_RS08630 (1749725) 1749725..1750276 + 552 WP_000102564.1 maltose O-acetyltransferase -
JJB13_RS08635 (1750392) 1750392..1750862 + 471 WP_000136192.1 YlaC family protein -
JJB13_RS08640 (1751026) 1751026..1752576 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JJB13_RS08645 (1752618) 1752618..1752971 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JJB13_RS08655 (1753350) 1753350..1753661 + 312 WP_000409911.1 MGMT family protein -
JJB13_RS08660 (1753692) 1753692..1754264 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T185987 WP_001291435.1 NZ_CP067265:1749335-1749553 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T185987 NZ_CP089135:c65007-64858 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACTGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT185987 WP_000344800.1 NZ_CP067265:1748935-1749309 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT185987 NZ_CP089135:65051-65112 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References