Detailed information of TA system TA185975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 15286..15929
• Replicon: plasmid pE25a
• Accession: NZ_CP067262
• Organism: Klebsiella variicola strain E25

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: JJB14_RS26680
• Protein ID: WP_208777956.1
• Coordinates: 15513..15929 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: JJB14_RS26675
• Protein ID: WP_001261276.1
• Coordinates: 15286..15516 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB14_RS26650 (JJB14_26640)	11433..12128	+	696	Protein_11	DNA-binding protein	-
JJB14_RS26655 (JJB14_26645)	12125..12745	+	621	WP_208777952.1	hypothetical protein	-
JJB14_RS27550	12742..12933	+	192	Protein_13	hypothetical protein	-
JJB14_RS26660 (JJB14_26650)	12949..13530	+	582	WP_227853092.1	DUF2971 domain-containing protein	-
JJB14_RS26665 (JJB14_26655)	13566..14306	+	741	WP_208777954.1	abortive infection family protein	-
JJB14_RS26670 (JJB14_26660)	14463..14792	-	330	WP_225315331.1	hypothetical protein	-
JJB14_RS26675 (JJB14_26665)	15286..15516	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JJB14_RS26680 (JJB14_26670)	15513..15929	+	417	WP_208777956.1	type II toxin-antitoxin system VapC family toxin	Toxin
JJB14_RS26685 (JJB14_26675)	16071..16288	+	218	Protein_19	hypothetical protein	-
JJB14_RS26690 (JJB14_26680)	16367..16693	+	327	WP_208777958.1	hypothetical protein	-
JJB14_RS26695 (JJB14_26685)	16916..18003	+	1088	Protein_21	transcriptional regulator	-
JJB14_RS26700 (JJB14_26690)	18004..20190	+	2187	WP_208777960.1	P-loop NTPase fold protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..98282	98282

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15020.55 Da
• Isoelectric Point: 9.2957

>T185975 WP_208777956.1 NZ_CP067262:15513-15929 [Klebsiella variicola]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVISAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T185975 NZ_CP089097:c4632261-4631728 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT185975 WP_001261276.1 NZ_CP067262:15286-15516 [Klebsiella variicola]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT185975 NZ_CP089097:c4632538-4632272 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A387K023