Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5401795..5402420 Replicon chromosome
Accession NZ_CP067261
Organism Klebsiella variicola strain E25

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag JJB14_RS26235 Protein ID WP_008807903.1
Coordinates 5401795..5402178 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JJB14_RS26240 Protein ID WP_004150355.1
Coordinates 5402178..5402420 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB14_RS26220 (JJB14_26210) 5399161..5400063 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JJB14_RS26225 (JJB14_26215) 5400060..5400695 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
JJB14_RS26230 (JJB14_26220) 5400692..5401621 + 930 WP_055323209.1 formate dehydrogenase accessory protein FdhE -
JJB14_RS26235 (JJB14_26225) 5401795..5402178 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
JJB14_RS26240 (JJB14_26230) 5402178..5402420 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
JJB14_RS26245 (JJB14_26235) 5402625..5403542 + 918 WP_208777668.1 alpha/beta hydrolase -
JJB14_RS26250 (JJB14_26240) 5403557..5404498 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
JJB14_RS26255 (JJB14_26245) 5404543..5404980 - 438 WP_012543288.1 D-aminoacyl-tRNA deacylase -
JJB14_RS26260 (JJB14_26250) 5404977..5405837 - 861 WP_008807907.1 virulence factor BrkB family protein -
JJB14_RS26265 (JJB14_26255) 5405831..5406430 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T185974 WP_008807903.1 NZ_CP067261:c5402178-5401795 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T185974 NZ_CP089097:4012381-4012599 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT185974 WP_004150355.1 NZ_CP067261:c5402420-5402178 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT185974 NZ_CP089097:4011980-4012354 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References