Detailed information of TA system TA185973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 5078615..5080296
• Replicon: chromosome
• Accession: NZ_CP067261
• Organism: Klebsiella variicola strain E25

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: JJB14_RS24740
• Protein ID: WP_022065798.1
• Coordinates: 5078615..5079532 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A0B7GCX3
• Locus tag: JJB14_RS24745
• Protein ID: WP_008807257.1
• Coordinates: 5079538..5080296 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB14_RS24720 (JJB14_24715)	5073921..5075441	+	1521	WP_008807253.1	sugar ABC transporter ATP-binding protein	-
JJB14_RS24725 (JJB14_24720)	5075438..5076436	+	999	WP_023296904.1	ribose ABC transporter permease	-
JJB14_RS24730 (JJB14_24725)	5076467..5077408	+	942	WP_012543155.1	ABC transporter substrate-binding protein	-
JJB14_RS24735 (JJB14_24730)	5077483..5078490	+	1008	WP_012969142.1	carbohydrate kinase family protein	-
JJB14_RS24740 (JJB14_24735)	5078615..5079532	-	918	WP_022065798.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
JJB14_RS24745 (JJB14_24740)	5079538..5080296	-	759	WP_008807257.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
JJB14_RS24750 (JJB14_24745)	5080381..5081109	-	729	WP_008807258.1	response regulator transcription factor	-
JJB14_RS24755 (JJB14_24750)	5081382..5083529	+	2148	WP_208777653.1	formate dehydrogenase subunit alpha	-
JJB14_RS24760 (JJB14_24755)	5084147..5084833	+	687	WP_008807260.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34751.14 Da
• Isoelectric Point: 5.4707

>T185973 WP_022065798.1 NZ_CP067261:c5079532-5078615 [Klebsiella variicola]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRTIALPHIRDALARIAANLQQQ
AGMNAVLQANRSDEMRVVVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELNRPIFNGFIAYLLSHNRPLAEVLSPRWKDIAEPFYREFSGMTFETIALEELTAVPYRMIDALKS
CFTQQDIDFLLSFKRGEPDWRLAPETRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T185973 NZ_CP089097:c3881457-3881140 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28653.01 Da
• Isoelectric Point: 10.1988

>AT185973 WP_008807257.1 NZ_CP067261:c5080296-5079538 [Klebsiella variicola]
MPSKLNWLLQNSAPGDVILQSWLSRHAISPSLAFKYTQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSMENKALLPKWFREFPGVEWIVISGQKLPVLDDKYRVTLDVKGKRLTGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQTKRLYLFFASFYEHGWLRRIDSQKIDLGAGKRQIVVNGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT185973 NZ_CP089097:c3881147-3880860 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTAGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B7GCX3