Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1798836..1799426 Replicon chromosome
Accession NZ_CP067261
Organism Klebsiella variicola strain E25

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag JJB14_RS08680 Protein ID WP_008804165.1
Coordinates 1799094..1799426 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag JJB14_RS08675 Protein ID WP_012541132.1
Coordinates 1798836..1799093 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB14_RS08655 (JJB14_08655) 1794444..1795019 + 576 WP_032733307.1 hypothetical protein -
JJB14_RS08660 (JJB14_08660) 1795197..1796033 + 837 WP_008804155.1 alpha/beta hydrolase -
JJB14_RS08665 (JJB14_08665) 1796239..1797210 + 972 WP_072124611.1 sensor domain-containing diguanylate cyclase -
JJB14_RS08670 (JJB14_08670) 1797207..1798307 - 1101 WP_048268588.1 AarF/UbiB family protein -
JJB14_RS08675 (JJB14_08675) 1798836..1799093 + 258 WP_012541132.1 antitoxin Antitoxin
JJB14_RS08680 (JJB14_08680) 1799094..1799426 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JJB14_RS08690 (JJB14_08690) 1799749..1801185 + 1437 WP_162494461.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
JJB14_RS08700 (JJB14_08700) 1801558..1803012 - 1455 WP_008804170.1 AMP nucleosidase -
JJB14_RS08705 (JJB14_08705) 1803143..1803388 - 246 WP_008804171.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T185960 WP_008804165.1 NZ_CP067261:1799094-1799426 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T185960 NZ_CP089094:62210-62548 [Microcystis aeruginosa FACHB-905 = DIANCHI905]
ATGACAATTTGGGTTGACGCACATTTGTCTCCTGCAATTGCAACCTGGATTAGCACTACACTTGAGATTGAAGTTGTGGC
TTTGCGTGATCTTGGACTGAGAGATGCTGAAGATACTGAAATCTTTCAGGTTGCAAAAGCTCAAAGAGCTATCCTAATGA
CCAAAGACAGCGATTTTGTAGATTTAGTTGAGCGACTTGGTTCGCCCCCACAAATTATTTGGCTGACTTGTGGCAATACC
TCTAATGCCCGGTTGCGTGAAATCTTGAGTGAGACTTTACCCAGGGCTTTAGAACTTTTAGCTGCTGGTGAGACATTGGT
AGAAATTAGCGGAGACTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT185960 WP_012541132.1 NZ_CP067261:1798836-1799093 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT185960 NZ_CP089094:61989-62213 [Microcystis aeruginosa FACHB-905 = DIANCHI905]
ATGGCGGAATTGCTTTCGAGAATTACAGTTAATCCTCGGCAGTGCGGTGGTCGTGCCTGTATTCGAGGAATGAGAATCCG
GGTATCAGATGTGTTAGATCTATTCGCGGCAGGTTTAAGCGCGAGAGAAATTCTAGATGAAATGCCTGATCTTGAGGCAG
ACGATTTGAAAGCGGCGCTTTTATATGCCTCGCGGAAGCTTAATCACCCGGTTTTACTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References