Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 35318..35961 Replicon plasmid pESBL_DR09b
Accession NZ_CP067257
Organism Klebsiella pneumoniae strain ESBL_DR09

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag JJB15_RS26915 Protein ID WP_000754566.1
Coordinates 35318..35734 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag JJB15_RS26920 Protein ID WP_001261276.1
Coordinates 35731..35961 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB15_RS26890 (JJB15_26895) 30318..31070 - 753 WP_000602738.1 IS6 family transposase -
JJB15_RS26895 (JJB15_26900) 31132..32013 + 882 Protein_35 Tn3 family transposase -
JJB15_RS26900 (JJB15_26905) 32176..32259 - 84 Protein_36 SOS response-associated peptidase -
JJB15_RS26905 (JJB15_26910) 32900..33976 - 1077 WP_000227969.1 IS110 family transposase -
JJB15_RS26910 (JJB15_26915) 34258..35114 - 857 Protein_38 IS3-like element ISEc15 family transposase -
JJB15_RS26915 (JJB15_26920) 35318..35734 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
JJB15_RS26920 (JJB15_26925) 35731..35961 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JJB15_RS26925 (JJB15_26930) 36535..36885 + 351 WP_000493378.1 hypothetical protein -
JJB15_RS26930 (JJB15_26935) 36936..37679 + 744 WP_000129823.1 hypothetical protein -
JJB15_RS26935 (JJB15_26940) 37676..38452 + 777 WP_000015958.1 site-specific integrase -
JJB15_RS26940 (JJB15_26945) 38510..38767 - 258 WP_000764642.1 hypothetical protein -
JJB15_RS26945 (JJB15_26950) 38896..39000 - 105 WP_032409716.1 hypothetical protein -
JJB15_RS26950 (JJB15_26955) 39530..40396 + 867 WP_004118283.1 replication initiation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid floR / tet(A) / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia / qnrB19 - 1..65548 65548


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T185955 WP_000754566.1 NZ_CP067257:c35734-35318 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T185955 NZ_CP089090:828767-829114 [Listeria marthii]
CTGATGGTGAAGCGTGGTGACGTATACTACGCGGACCTTTCCCCCGTGGTCGGAAGCGAGCAAGGGGGAATAAGGCCTGT
TCTCATCATTCAAAATGATATTGGAAATCGATTCAGTCCAACTGTGATTGTGGCAGCAATAACTGCAAAGATTCAAAAAG
CAAAACTGCCAACTCACGTGGAAGCTACTCGTAAAGATGGCTTTGAGAGAGATTCTGTCATTCTTTTGGAACAAATTAGA
ACGATTGACAAACAGCGCCTGACAGATAAAATTACACATTTGGACGAAGAGTTAATGGCCAAGGTAAACAAGGCACTCGA
AGTTAGTCTAGGAGTAGTAGAATTCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT185955 WP_001261276.1 NZ_CP067257:c35961-35731 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT185955 NZ_CP089090:828485-828763 [Listeria marthii]
GTGTTAGAGAAAGAAAAGCGGATGATAATATCCGTAGAACTGACACAGGAAATGGTACAAGAACTCGACGTAGTTGTAGA
AAAAGAAAAAATGGGGCGGAGTGAAGTTATAATGGAAGCAACGCAACAATTTTTACAAGAGAAAAGGGCTCGCGAATTAA
GAGACGAGATGGAACGCGGTTATGCAGAAATGGCGACAATTAATTTCGCTATTGCATGCGAGTGTACTCATGTCGAGGCA
GAAGCAGAAGACAGGAATATTAGTATTTTAGGAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References