Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 53674..54410 Replicon plasmid pESBL_DR09a
Accession NZ_CP067256
Organism Klebsiella pneumoniae strain ESBL_DR09

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag JJB15_RS26085 Protein ID WP_003026803.1
Coordinates 53928..54410 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag JJB15_RS26080 Protein ID WP_003026799.1
Coordinates 53674..53940 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB15_RS26035 (JJB15_26040) 49736..50098 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
JJB15_RS26040 (JJB15_26045) 50148..50498 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
JJB15_RS26045 (JJB15_26050) 50856..51125 + 270 WP_004152102.1 hypothetical protein -
JJB15_RS26050 (JJB15_26055) 51113..51688 + 576 WP_004152103.1 hypothetical protein -
JJB15_RS26055 (JJB15_26060) 51719..52213 + 495 WP_004152104.1 DNA-binding protein -
JJB15_RS26060 (JJB15_26065) 52257..52625 + 369 WP_004152105.1 hypothetical protein -
JJB15_RS26065 (JJB15_26070) 52659..52862 + 204 WP_004152106.1 HHA domain-containing protein -
JJB15_RS26070 (JJB15_26075) 52911..53168 + 258 WP_004152107.1 hypothetical protein -
JJB15_RS26075 (JJB15_26080) 53244..53498 + 255 WP_004152108.1 hypothetical protein -
JJB15_RS26080 (JJB15_26085) 53674..53940 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
JJB15_RS26085 (JJB15_26090) 53928..54410 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
JJB15_RS26090 (JJB15_26095) 54618..55964 + 1347 WP_077253535.1 ISNCY family transposase -
JJB15_RS26095 (JJB15_26100) 56013..56408 + 396 WP_004143398.1 helix-turn-helix domain-containing protein -
JJB15_RS26100 (JJB15_26105) 56556..57721 - 1166 Protein_59 IS3 family transposase -
JJB15_RS26105 (JJB15_26110) 57898..58860 - 963 WP_004152113.1 zinc metalloprotease HtpX -
JJB15_RS26110 (JJB15_26115) 58847..59335 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / dfrA14 - 1..172022 172022


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T185954 WP_003026803.1 NZ_CP067256:53928-54410 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T185954 NZ_CP089089:955874-956221 [Listeria marthii]
CTGATGGTGAAGCGTGGTGACGTATACTACGCGGACCTTTCCCCCGTGGTCGGAAGCGAGCAAGGGGGAATAAGGCCTGT
TCTCATCATTCAAAATGATATTGGAAATCGATTCAGTCCAACTGTGATTGTGGCAGCAATAACTGCAAAGATTCAAAAAG
CAAAACTGCCAACTCACGTGGAAGCTACTCGTAAAGATGGCTTTGAGAGAGATTCTGTCATTCTTTTGGAACAAATTAGA
ACGATTGACAAACAGCGCCTGACAGATAAAATTACACATTTGGACGAAGAGTTAATGGCCAAGGTAAACAAGGCACTCGA
AGTTAGTCTAGGAGTAGTAGAATTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT185954 WP_003026799.1 NZ_CP067256:53674-53940 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT185954 NZ_CP089089:955592-955870 [Listeria marthii]
GTGTTAGAGAAAGAAAAGCGGATGATAATATCCGTAGAACTGACACAGGAAATGGTACAAGAACTCGACGTAGTTGTAGA
AAAAGAAAAAATGGGGCGGAGTGAAGTTATAATGGAAGCAACGCAACAATTTTTACAAGAGAAAAGGGCTCGCGAATTAA
GAGACGAGATGGAACGCGGTTATGCAGAAATGGCGACAATTAATTTCGCTATTGCATGCGAGTGTACTCATGTCGAAGCA
GAAGCAGAAGACAGGAATATTAGTATTTTAGGAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References